Cargando…

Silencing of Transposable Elements Mediated by 5-mC and Compensation of the Heterochromatin Content by Presence of B Chromosomes in Astyanax scabripinnis

The way in which transcriptional activity overcomes the physical DNA structure and gene regulation mechanisms involves complex processes that are not yet fully understood. Modifications in the cytosine-guanine sequence of DNA by 5-mC are preferentially located in heterochromatic regions and are rela...

Descripción completa

Detalles Bibliográficos
Autores principales: Barbosa, Patrícia, Schemczssen-Graeff, Zelinda, Marques, André, da Silva, Maelin, Favero, Giovani Marino, Sobreiro, Bernardo Passos, de Almeida, Mara Cristina, Moreira-Filho, Orlando, Silva, Duílio Mazzoni Zerbinato de Andrade, Porto-Foresti, Fábio, Foresti, Fausto, Artoni, Roberto Ferreira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151356/
https://www.ncbi.nlm.nih.gov/pubmed/34064768
http://dx.doi.org/10.3390/cells10051162
_version_ 1783698363723546624
author Barbosa, Patrícia
Schemczssen-Graeff, Zelinda
Marques, André
da Silva, Maelin
Favero, Giovani Marino
Sobreiro, Bernardo Passos
de Almeida, Mara Cristina
Moreira-Filho, Orlando
Silva, Duílio Mazzoni Zerbinato de Andrade
Porto-Foresti, Fábio
Foresti, Fausto
Artoni, Roberto Ferreira
author_facet Barbosa, Patrícia
Schemczssen-Graeff, Zelinda
Marques, André
da Silva, Maelin
Favero, Giovani Marino
Sobreiro, Bernardo Passos
de Almeida, Mara Cristina
Moreira-Filho, Orlando
Silva, Duílio Mazzoni Zerbinato de Andrade
Porto-Foresti, Fábio
Foresti, Fausto
Artoni, Roberto Ferreira
author_sort Barbosa, Patrícia
collection PubMed
description The way in which transcriptional activity overcomes the physical DNA structure and gene regulation mechanisms involves complex processes that are not yet fully understood. Modifications in the cytosine-guanine sequence of DNA by 5-mC are preferentially located in heterochromatic regions and are related to gene silencing. Herein, we investigate evidence of epigenetic regulation related to the B chromosome model and transposable elements in A. scabripinnis. Indirect immunofluorescence using anti-5-mC to mark methylated regions was employed along with quantitative ELISA to determine the total genomic DNA methylation level. 5-mC signals were dispersed in the chromosomes of both females and males, with preferential accumulation in the B chromosome. In addition to the heterochromatic methylated regions, our results suggest that methylation is associated with transposable elements (LINE and Tc1-Mariner). Heterochromatin content was measured based on the C-band length in relation to the size of chromosome 1. The B chromosome in A. scabripinnis comprises heterochromatin located in the pericentromeric region of both arms of this isochromosome. In this context, individuals with B chromosomes should have an increased heterochromatin content when compared to individuals that do not. Although, both heterochromatin content and genome methylation showed no significant differences between sexes or in relation to the occurrence of B chromosomes. Our evidence suggests that the B chromosome can have a compensation effect on the heterochromatin content and that methylation possibly operates to silence TEs in A. scabripinnis. This represents a sui generis compensation and gene activity buffering mechanism.
format Online
Article
Text
id pubmed-8151356
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81513562021-05-27 Silencing of Transposable Elements Mediated by 5-mC and Compensation of the Heterochromatin Content by Presence of B Chromosomes in Astyanax scabripinnis Barbosa, Patrícia Schemczssen-Graeff, Zelinda Marques, André da Silva, Maelin Favero, Giovani Marino Sobreiro, Bernardo Passos de Almeida, Mara Cristina Moreira-Filho, Orlando Silva, Duílio Mazzoni Zerbinato de Andrade Porto-Foresti, Fábio Foresti, Fausto Artoni, Roberto Ferreira Cells Article The way in which transcriptional activity overcomes the physical DNA structure and gene regulation mechanisms involves complex processes that are not yet fully understood. Modifications in the cytosine-guanine sequence of DNA by 5-mC are preferentially located in heterochromatic regions and are related to gene silencing. Herein, we investigate evidence of epigenetic regulation related to the B chromosome model and transposable elements in A. scabripinnis. Indirect immunofluorescence using anti-5-mC to mark methylated regions was employed along with quantitative ELISA to determine the total genomic DNA methylation level. 5-mC signals were dispersed in the chromosomes of both females and males, with preferential accumulation in the B chromosome. In addition to the heterochromatic methylated regions, our results suggest that methylation is associated with transposable elements (LINE and Tc1-Mariner). Heterochromatin content was measured based on the C-band length in relation to the size of chromosome 1. The B chromosome in A. scabripinnis comprises heterochromatin located in the pericentromeric region of both arms of this isochromosome. In this context, individuals with B chromosomes should have an increased heterochromatin content when compared to individuals that do not. Although, both heterochromatin content and genome methylation showed no significant differences between sexes or in relation to the occurrence of B chromosomes. Our evidence suggests that the B chromosome can have a compensation effect on the heterochromatin content and that methylation possibly operates to silence TEs in A. scabripinnis. This represents a sui generis compensation and gene activity buffering mechanism. MDPI 2021-05-11 /pmc/articles/PMC8151356/ /pubmed/34064768 http://dx.doi.org/10.3390/cells10051162 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Barbosa, Patrícia
Schemczssen-Graeff, Zelinda
Marques, André
da Silva, Maelin
Favero, Giovani Marino
Sobreiro, Bernardo Passos
de Almeida, Mara Cristina
Moreira-Filho, Orlando
Silva, Duílio Mazzoni Zerbinato de Andrade
Porto-Foresti, Fábio
Foresti, Fausto
Artoni, Roberto Ferreira
Silencing of Transposable Elements Mediated by 5-mC and Compensation of the Heterochromatin Content by Presence of B Chromosomes in Astyanax scabripinnis
title Silencing of Transposable Elements Mediated by 5-mC and Compensation of the Heterochromatin Content by Presence of B Chromosomes in Astyanax scabripinnis
title_full Silencing of Transposable Elements Mediated by 5-mC and Compensation of the Heterochromatin Content by Presence of B Chromosomes in Astyanax scabripinnis
title_fullStr Silencing of Transposable Elements Mediated by 5-mC and Compensation of the Heterochromatin Content by Presence of B Chromosomes in Astyanax scabripinnis
title_full_unstemmed Silencing of Transposable Elements Mediated by 5-mC and Compensation of the Heterochromatin Content by Presence of B Chromosomes in Astyanax scabripinnis
title_short Silencing of Transposable Elements Mediated by 5-mC and Compensation of the Heterochromatin Content by Presence of B Chromosomes in Astyanax scabripinnis
title_sort silencing of transposable elements mediated by 5-mc and compensation of the heterochromatin content by presence of b chromosomes in astyanax scabripinnis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151356/
https://www.ncbi.nlm.nih.gov/pubmed/34064768
http://dx.doi.org/10.3390/cells10051162
work_keys_str_mv AT barbosapatricia silencingoftransposableelementsmediatedby5mcandcompensationoftheheterochromatincontentbypresenceofbchromosomesinastyanaxscabripinnis
AT schemczssengraeffzelinda silencingoftransposableelementsmediatedby5mcandcompensationoftheheterochromatincontentbypresenceofbchromosomesinastyanaxscabripinnis
AT marquesandre silencingoftransposableelementsmediatedby5mcandcompensationoftheheterochromatincontentbypresenceofbchromosomesinastyanaxscabripinnis
AT dasilvamaelin silencingoftransposableelementsmediatedby5mcandcompensationoftheheterochromatincontentbypresenceofbchromosomesinastyanaxscabripinnis
AT faverogiovanimarino silencingoftransposableelementsmediatedby5mcandcompensationoftheheterochromatincontentbypresenceofbchromosomesinastyanaxscabripinnis
AT sobreirobernardopassos silencingoftransposableelementsmediatedby5mcandcompensationoftheheterochromatincontentbypresenceofbchromosomesinastyanaxscabripinnis
AT dealmeidamaracristina silencingoftransposableelementsmediatedby5mcandcompensationoftheheterochromatincontentbypresenceofbchromosomesinastyanaxscabripinnis
AT moreirafilhoorlando silencingoftransposableelementsmediatedby5mcandcompensationoftheheterochromatincontentbypresenceofbchromosomesinastyanaxscabripinnis
AT silvaduiliomazzonizerbinatodeandrade silencingoftransposableelementsmediatedby5mcandcompensationoftheheterochromatincontentbypresenceofbchromosomesinastyanaxscabripinnis
AT portoforestifabio silencingoftransposableelementsmediatedby5mcandcompensationoftheheterochromatincontentbypresenceofbchromosomesinastyanaxscabripinnis
AT forestifausto silencingoftransposableelementsmediatedby5mcandcompensationoftheheterochromatincontentbypresenceofbchromosomesinastyanaxscabripinnis
AT artonirobertoferreira silencingoftransposableelementsmediatedby5mcandcompensationoftheheterochromatincontentbypresenceofbchromosomesinastyanaxscabripinnis