Early Heat Exposure Effects on Proteomic Changes of the Broiler Liver under Acute Heat Stress

SIMPLE SUMMARY: Early heat exposure have been studied in the poultry industry as a method of reducing heat stress (HS) on poultry. However, the results of each study are inconsistent, and it has not been confirmed which mechanisms reduce HS by early heat exposure. Therefore, we tried to confirm the relaxation mechanism through proteomic analysis after applying early and acute heat exposure to broilers. The broilers were divided into three treatments, followed by CC (control group), CH (acute HS at the 35th day), and HH (early heat exposure at the fifth day and acute HS at the 35th day. Liver samples were collected and analyzed for proteomics and functional analysis. Proteins related to various functions, such as carbohydrate metabolism, fatty acid metabolism, energy metabolism, and the oxidation–reduction process, which were dramatically changed by acute HS, and were alleviated similar to the control group by early heat exposure. Through these results, the mechanism by which early heat exposure induces homeostasis during acute HS, and the possibility of the early heat exposure as a method of reducing HS were confirmed. ABSTRACT: As environmental temperatures continue to rise, heat stress (HS) is having a negative effect on the livestock industry. In order to solve this problem, many studies have been conducted to reduce HS. Among them, early heat exposure has been suggested as a method for reducing HS in poultry. In this study, we analyzed proteomics and tried to identify the metabolic mechanisms of early heat exposure on acute HS. A total of 48 chicks were separated into three groups: CC (control groups raised at optimum temperature), CH (raised with CC but exposed acute HS at the 35th day), and HH (raised with CC but exposed early heat at the fifth day and acute HS at the 35th day). After the whole period, liver samples were collected for proteomic analysis. A total of 97 differentially expressed proteins were identified by acute HS. Of these, 62 proteins recovered their expression levels by early heat exposure. We used these 62 proteins to determine the protective effects of early heat exposure. Of the various protein-related terms, we focused on the oxidative phosphorylation, fatty acid metabolism, carbohydrate metabolism, and energy production metabolism. Our findings suggest the possibility of early heat exposure effects in acute HS that may be useful in breeding or management techniques for producing broilers with high heat resistance.