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Making, Cloning, and the Expression of Human Insulin Genes in Bacteria: The Path to Humulin
In the mid- to late 1970s, recombinant deoxyribonucleic acid methods for cloning and expressing genes in E. coli were under intense development. The important question had become: Can humans design and chemically synthesize novel genes that function in bacteria? This question was answered in 1978 an...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Oxford University Press
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152450/ https://www.ncbi.nlm.nih.gov/pubmed/33340315 http://dx.doi.org/10.1210/endrev/bnaa029 |