Rapid no-wash labeling of PYP-tag proteins with reactive fluorogenic ligands affords stable fluorescent protein conjugates for long-term cell imaging studies

Covalent labeling systems that employ protein-tags or chemical probes to convert proteins into fluorescent conjugates are powerful tools for carrying out real time imaging and pulse-chase tracking studies that enable the spatiotemporal role of proteins in complex biological systems to be investigate...

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Detalles Bibliográficos
Autores principales: Kumar, Naresh, Hori, Yuichiro, Nishiura, Miyako, Kikuchi, Kazuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152630/
https://www.ncbi.nlm.nih.gov/pubmed/34094058
http://dx.doi.org/10.1039/d0sc00499e

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152630/
https://www.ncbi.nlm.nih.gov/pubmed/34094058
http://dx.doi.org/10.1039/d0sc00499e

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