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Metabolome and Transcriptome Integration Reveals Insights Into Flavor Formation of ‘Crimson’ Watermelon Flesh During Fruit Development

Metabolites have been reported as the main factor that influences the fruit flavor of watermelon. But the comprehensive study on the dynamics of metabolites during the development of watermelon fruit is not up-to-date. In this study, metabolome and transcriptome datasets of ‘Crimson’ watermelon frui...

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Autores principales: Gong, Chengsheng, Diao, Weinan, Zhu, Hongju, Umer, Muhammad Jawad, Zhao, Shengjie, He, Nan, Lu, Xuqiang, Yuan, Pingli, Anees, Muhammad, Yang, Dongdong, Kaseb, M. O., Liu, Wenge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153042/
https://www.ncbi.nlm.nih.gov/pubmed/34054886
http://dx.doi.org/10.3389/fpls.2021.629361
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author Gong, Chengsheng
Diao, Weinan
Zhu, Hongju
Umer, Muhammad Jawad
Zhao, Shengjie
He, Nan
Lu, Xuqiang
Yuan, Pingli
Anees, Muhammad
Yang, Dongdong
Kaseb, M. O.
Liu, Wenge
author_facet Gong, Chengsheng
Diao, Weinan
Zhu, Hongju
Umer, Muhammad Jawad
Zhao, Shengjie
He, Nan
Lu, Xuqiang
Yuan, Pingli
Anees, Muhammad
Yang, Dongdong
Kaseb, M. O.
Liu, Wenge
author_sort Gong, Chengsheng
collection PubMed
description Metabolites have been reported as the main factor that influences the fruit flavor of watermelon. But the comprehensive study on the dynamics of metabolites during the development of watermelon fruit is not up-to-date. In this study, metabolome and transcriptome datasets of ‘Crimson’ watermelon fruit at four key developmental stages were generated. A total of 517 metabolites were detected by ultrahigh-performance liquid chromatography–electrospray ionization–tandem mass spectrometry and gas chromatography–solid-phase microextraction–mass spectrometry. Meanwhile, by K-means clustering analysis, the total differentially expressed genes were clustered in six classes. Integrating transcriptome and metabolome data revealed similar expression trends of sugars and genes involved in the glycolytic pathway, providing molecular insights into the formation of taste during fruit development. Furthermore, through coexpression analysis, we identified five differentially expressed ADH (alcohol dehydrogenase) genes (Cla97C01G013600, Cla97C05G089700, Cla97C01G001290, Cla97C05G095170, and Cla97C06G118330), which were found to be closely related to C9 alcohols/aldehydes, providing information for the formation of fruit aroma. Our findings establish a metabolic profile during watermelon fruit development and provide insights into flavor formation.
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spelling pubmed-81530422021-05-27 Metabolome and Transcriptome Integration Reveals Insights Into Flavor Formation of ‘Crimson’ Watermelon Flesh During Fruit Development Gong, Chengsheng Diao, Weinan Zhu, Hongju Umer, Muhammad Jawad Zhao, Shengjie He, Nan Lu, Xuqiang Yuan, Pingli Anees, Muhammad Yang, Dongdong Kaseb, M. O. Liu, Wenge Front Plant Sci Plant Science Metabolites have been reported as the main factor that influences the fruit flavor of watermelon. But the comprehensive study on the dynamics of metabolites during the development of watermelon fruit is not up-to-date. In this study, metabolome and transcriptome datasets of ‘Crimson’ watermelon fruit at four key developmental stages were generated. A total of 517 metabolites were detected by ultrahigh-performance liquid chromatography–electrospray ionization–tandem mass spectrometry and gas chromatography–solid-phase microextraction–mass spectrometry. Meanwhile, by K-means clustering analysis, the total differentially expressed genes were clustered in six classes. Integrating transcriptome and metabolome data revealed similar expression trends of sugars and genes involved in the glycolytic pathway, providing molecular insights into the formation of taste during fruit development. Furthermore, through coexpression analysis, we identified five differentially expressed ADH (alcohol dehydrogenase) genes (Cla97C01G013600, Cla97C05G089700, Cla97C01G001290, Cla97C05G095170, and Cla97C06G118330), which were found to be closely related to C9 alcohols/aldehydes, providing information for the formation of fruit aroma. Our findings establish a metabolic profile during watermelon fruit development and provide insights into flavor formation. Frontiers Media S.A. 2021-05-12 /pmc/articles/PMC8153042/ /pubmed/34054886 http://dx.doi.org/10.3389/fpls.2021.629361 Text en Copyright © 2021 Gong, Diao, Zhu, Umer, Zhao, He, Lu, Yuan, Anees, Yang, Kaseb and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Gong, Chengsheng
Diao, Weinan
Zhu, Hongju
Umer, Muhammad Jawad
Zhao, Shengjie
He, Nan
Lu, Xuqiang
Yuan, Pingli
Anees, Muhammad
Yang, Dongdong
Kaseb, M. O.
Liu, Wenge
Metabolome and Transcriptome Integration Reveals Insights Into Flavor Formation of ‘Crimson’ Watermelon Flesh During Fruit Development
title Metabolome and Transcriptome Integration Reveals Insights Into Flavor Formation of ‘Crimson’ Watermelon Flesh During Fruit Development
title_full Metabolome and Transcriptome Integration Reveals Insights Into Flavor Formation of ‘Crimson’ Watermelon Flesh During Fruit Development
title_fullStr Metabolome and Transcriptome Integration Reveals Insights Into Flavor Formation of ‘Crimson’ Watermelon Flesh During Fruit Development
title_full_unstemmed Metabolome and Transcriptome Integration Reveals Insights Into Flavor Formation of ‘Crimson’ Watermelon Flesh During Fruit Development
title_short Metabolome and Transcriptome Integration Reveals Insights Into Flavor Formation of ‘Crimson’ Watermelon Flesh During Fruit Development
title_sort metabolome and transcriptome integration reveals insights into flavor formation of ‘crimson’ watermelon flesh during fruit development
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153042/
https://www.ncbi.nlm.nih.gov/pubmed/34054886
http://dx.doi.org/10.3389/fpls.2021.629361
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