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DNA methylation analysis: speedup of bisulfite-mediated deamination of cytosine in the genomic sequencing procedure

Understanding the biological consequences of DNA methylation is a current focus of intensive studies. A standard method for analyzing the methylation at position 5 of cytosines in genomic DNA involves chemical modification of the DNA with bisulfite, followed by PCR amplification and sequencing. Bisu...

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Detalles Bibliográficos
Autores principales: Hayatsu, Hikoya, Negishi, Kazuo, Shiraishi, Masahiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japan Academy 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153359/