Assessment of TMT Labeling Efficiency in Large-Scale Quantitative Proteomics: The Critical Effect of Sample pH

[Image: see text] Isobaric labeling via tandem mass tag (TMT) reagents enables sample multiplexing prior to LC–MS/MS, facilitating high-throughput large-scale quantitative proteomics. Consistent and efficient labeling reactions are essential to achieve robust quantification; therefore, embedded in o...

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Detalles Bibliográficos
Autores principales: Hutchinson-Bunch, Chelsea, Sanford, James A., Hansen, Joshua R., Gritsenko, Marina A., Rodland, Karin D., Piehowski, Paul D., Qian, Wei-Jun, Adkins, Joshua N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154127/
https://www.ncbi.nlm.nih.gov/pubmed/34056417
http://dx.doi.org/10.1021/acsomega.1c00776

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154127/
https://www.ncbi.nlm.nih.gov/pubmed/34056417
http://dx.doi.org/10.1021/acsomega.1c00776

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