Cargando…

Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single gene

The carboxyl-terminal domain of RNA polymerase II (RNAP2) is phosphorylated during transcription in eukaryotic cells. While residue-specific phosphorylation has been mapped with exquisite spatial resolution along the 1D genome in a population of fixed cells using immunoprecipitation-based assays, th...

Descripción completa

Detalles Bibliográficos
Autores principales:	Forero-Quintero, Linda S., Raymond, William, Handa, Tetsuya, Saxton, Matthew N., Morisaki, Tatsuya, Kimura, Hiroshi, Bertrand, Edouard, Munsky, Brian, Stasevich, Timothy J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155019/ https://www.ncbi.nlm.nih.gov/pubmed/34039974 http://dx.doi.org/10.1038/s41467-021-23417-0

Ejemplares similares

Live-cell imaging uncovers the relationship between histone acetylation, transcription initiation, and nucleosome mobility
por: Saxton, Matthew N., et al.
Publicado: (2023)

Computational design and interpretation of single-RNA translation experiments
por: Aguilera, Luis U., et al.
Publicado: (2019)

Using mechanistic models and machine learning to design single-color multiplexed Nascent Chain Tracking experiments
por: Raymond, William S., et al.
Publicado: (2023)

Using mechanistic models and machine learning to design single-color multiplexed nascent chain tracking experiments
por: Raymond, William S., et al.
Publicado: (2023)

Live imaging of transcription sites using an elongating RNA polymerase II–specific probe
por: Uchino, Satoshi, et al.
Publicado: (2021)

Visualizing posttranslational and epigenetic modifications of endogenous proteins in vivo
por: Kimura, Hiroshi, et al.
Publicado: (2015)

Analysis and design of single-cell experiments to harvest fluctuation information while rejecting measurement noise
por: Vo, Huy D., et al.
Publicado: (2023)

Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase
por: Hayashi-Takanaka, Yoko, et al.
Publicado: (2009)

Imaging translational control by Argonaute with single-molecule resolution in live cells
por: Cialek, Charlotte A., et al.
Publicado: (2022)

Tracking epigenetic histone modifications in single cells using Fab-based live endogenous modification labeling
por: Hayashi-Takanaka, Yoko, et al.
Publicado: (2011)

A live imaging system to analyze spatiotemporal dynamics of RNA polymerase II modification in Arabidopsis thaliana
por: Shibuta, Mio K., et al.
Publicado: (2021)

Visualizing looping of two endogenous genomic loci using synthetic zinc‐finger proteins with anti‐FLAG and anti‐HA frankenbodies in living cells
por: Liu, Yang, et al.
Publicado: (2021)

A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo
por: Zhao, Ning, et al.
Publicado: (2019)

Coupling of translation quality control and mRNA targeting to stress granules
por: Moon, Stephanie L., et al.
Publicado: (2020)

Evaluation of Chemical Fluorescent Dyes as a Protein Conjugation Partner for Live Cell Imaging
por: Hayashi-Takanaka, Yoko, et al.
Publicado: (2014)

The transcription cycle of RNA polymerase II in living cells
por: Kimura, Hiroshi, et al.
Publicado: (2002)

Endogenous overexpression of an active phosphorylated form of DNA polymerase β under oxidative stress in Trypanosoma cruzi
por: Rojas, Diego A., et al.
Publicado: (2018)

Multicolor single-molecule tracking of mRNA interactions with RNP granules
por: Moon, Stephanie L., et al.
Publicado: (2019)

Visualization of single endogenous polysomes reveals the dynamics of translation in live human cells
por: Pichon, Xavier, et al.
Publicado: (2016)

Spatiotemporal Regulation of Metal Ions in the Polymerase Chain Reaction
por: Zhang, Xianjing, et al.
Publicado: (2022)

She1 affects dynein through direct interactions with the microtubule and the dynein microtubule-binding domain
por: Ecklund, Kari H., et al.
Publicado: (2017)

RNA polymerase II condensate formation and association with Cajal and histone locus bodies in living human cells
por: Imada, Takashi, et al.
Publicado: (2021)

DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast
por: Handa, Tetsuya, et al.
Publicado: (2012)

Site specific phosphorylation of yeast RNA polymerase I
por: Gerber, Jochen, et al.
Publicado: (2008)

Structure of TFIIK for phosphorylation of CTD of RNA polymerase II
por: van Eeuwen, Trevor, et al.
Publicado: (2021)

Phosphorylation Induced Conformational Transitions in DNA Polymerase β
por: Srivastava, Amit, et al.
Publicado: (2022)

Live-cell analysis of endogenous GFP-RPB1 uncovers rapid turnover of initiating and promoter-paused RNA Polymerase II
por: Steurer, Barbara, et al.
Publicado: (2018)

Spatiotemporal dynamics of dendritic spines in the living brain
por: Chen, Chia-Chien, et al.
Publicado: (2014)

Structural Insights into Phosphorylation-Mediated Polymerase Function Loss for DNA Polymerase β Bound to Gapped DNA
por: Srivastava, Amit, et al.
Publicado: (2023)

LOCALIZATION OF ENDOGENOUS RNA POLYMERASE ACTIVITY IN FROZEN SECTIONS OF PLANT TISSUES
por: Fisher, Donald B.
Publicado: (1968)

Spatiotemporal variation of endogenous cell-generated stresses within 3D multicellular spheroids
por: Lucio, Adam A., et al.
Publicado: (2017)

Regulation of RNA Polymerase II Termination by Phosphorylation of Gdown1
por: Guo, Jiannan, et al.
Publicado: (2014)

Phosphorylation controls RNA binding and transcription by the influenza virus polymerase
por: Dawson, Anthony R., et al.
Publicado: (2020)

Spatially coordinated collective phosphorylation filters spatiotemporal noises for precise circadian timekeeping
por: Chae, Seok Joo, et al.
Publicado: (2023)

Super-resolution imaging of fluorescently labeled, endogenous RNA Polymerase II in living cells with CRISPR/Cas9-mediated gene editing
por: Cho, Won-Ki, et al.
Publicado: (2016)

A benchmark for chromatin binding measurements in live cells
por: Mazza, Davide, et al.
Publicado: (2012)

Compartmentalization of androgen receptors at endogenous genes in living cells
por: Yavuz, Selçuk, et al.
Publicado: (2023)

Phosphorylation of the PA subunit of influenza polymerase at Y393 prevents binding of the 5′-termini of RNA and polymerase function
por: Liu, Lu, et al.
Publicado: (2023)

Endogenous DAF-16 spatiotemporal activity quantitatively predicts lifespan extension induced by dietary restriction
por: Huayta, Javier, et al.
Publicado: (2023)

A phosphorylation switch controls the spatiotemporal activation of Rho GTPases in directional cell migration
por: Cao, Xuan, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_51qmv7d7o8idu110m5ojha3lmd