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Detection of Chilling Injury in Pickling Cucumbers Using Dual-Band Chlorophyll Fluorescence Imaging

Pickling cucumbers are susceptible to chilling injury (CI) during postharvest refrigerated storage, which would result in quality degradation and economic loss. It is, thus, desirable to remove the defective fruit before they are marketed as fresh products or processed into pickled products. Chlorop...

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Autores principales: Lu, Yuzhen, Lu, Renfu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156177/
https://www.ncbi.nlm.nih.gov/pubmed/34069201
http://dx.doi.org/10.3390/foods10051094
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author Lu, Yuzhen
Lu, Renfu
author_facet Lu, Yuzhen
Lu, Renfu
author_sort Lu, Yuzhen
collection PubMed
description Pickling cucumbers are susceptible to chilling injury (CI) during postharvest refrigerated storage, which would result in quality degradation and economic loss. It is, thus, desirable to remove the defective fruit before they are marketed as fresh products or processed into pickled products. Chlorophyll fluorescence is sensitive to CI in green fruits, because exposure to chilling temperatures can induce detectable alterations in chlorophylls of tissues. This study evaluated the feasibility of using a dual-band chlorophyll fluorescence imaging (CFI) technique for detecting CI-affected pickling cucumbers. Chlorophyll fluorescence images at 675 nm and 750 nm were acquired from pickling cucumbers under the excitation of ultraviolet-blue light. The raw images were processed for vignetting corrections through bi-dimensional empirical mode decomposition and subsequent image reconstruction. The fluorescence images were effective for ascertaining CI-affected tissues, which appeared as dark areas in the images. Support vector machine models were developed for classifying pickling cucumbers into two or three classes using the features extracted from the fluorescence images. Fusing the features of fluorescence images at 675 nm and 750 nm resulted in overall accuracies of 96.9% and 91.2% for two-class (normal and injured) and three-class (normal, mildly and severely injured) classification, respectively, which are statistically significantly better than those obtained using the features at a single wavelength, especially for the three-class classification. Furthermore, a subset of features, selected based on the neighborhood component feature selection technique, achieved the highest accuracies of 97.4% and 91.3% for the two-class and three-class classification, respectively. This study demonstrated that dual-band CFI is an effective modality for CI detection in pickling cucumbers.
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spelling pubmed-81561772021-05-28 Detection of Chilling Injury in Pickling Cucumbers Using Dual-Band Chlorophyll Fluorescence Imaging Lu, Yuzhen Lu, Renfu Foods Article Pickling cucumbers are susceptible to chilling injury (CI) during postharvest refrigerated storage, which would result in quality degradation and economic loss. It is, thus, desirable to remove the defective fruit before they are marketed as fresh products or processed into pickled products. Chlorophyll fluorescence is sensitive to CI in green fruits, because exposure to chilling temperatures can induce detectable alterations in chlorophylls of tissues. This study evaluated the feasibility of using a dual-band chlorophyll fluorescence imaging (CFI) technique for detecting CI-affected pickling cucumbers. Chlorophyll fluorescence images at 675 nm and 750 nm were acquired from pickling cucumbers under the excitation of ultraviolet-blue light. The raw images were processed for vignetting corrections through bi-dimensional empirical mode decomposition and subsequent image reconstruction. The fluorescence images were effective for ascertaining CI-affected tissues, which appeared as dark areas in the images. Support vector machine models were developed for classifying pickling cucumbers into two or three classes using the features extracted from the fluorescence images. Fusing the features of fluorescence images at 675 nm and 750 nm resulted in overall accuracies of 96.9% and 91.2% for two-class (normal and injured) and three-class (normal, mildly and severely injured) classification, respectively, which are statistically significantly better than those obtained using the features at a single wavelength, especially for the three-class classification. Furthermore, a subset of features, selected based on the neighborhood component feature selection technique, achieved the highest accuracies of 97.4% and 91.3% for the two-class and three-class classification, respectively. This study demonstrated that dual-band CFI is an effective modality for CI detection in pickling cucumbers. MDPI 2021-05-14 /pmc/articles/PMC8156177/ /pubmed/34069201 http://dx.doi.org/10.3390/foods10051094 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lu, Yuzhen
Lu, Renfu
Detection of Chilling Injury in Pickling Cucumbers Using Dual-Band Chlorophyll Fluorescence Imaging
title Detection of Chilling Injury in Pickling Cucumbers Using Dual-Band Chlorophyll Fluorescence Imaging
title_full Detection of Chilling Injury in Pickling Cucumbers Using Dual-Band Chlorophyll Fluorescence Imaging
title_fullStr Detection of Chilling Injury in Pickling Cucumbers Using Dual-Band Chlorophyll Fluorescence Imaging
title_full_unstemmed Detection of Chilling Injury in Pickling Cucumbers Using Dual-Band Chlorophyll Fluorescence Imaging
title_short Detection of Chilling Injury in Pickling Cucumbers Using Dual-Band Chlorophyll Fluorescence Imaging
title_sort detection of chilling injury in pickling cucumbers using dual-band chlorophyll fluorescence imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156177/
https://www.ncbi.nlm.nih.gov/pubmed/34069201
http://dx.doi.org/10.3390/foods10051094
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