Knock-in and precise nucleotide substitution using near-PAMless engineered Cas9 variants in Dictyostelium discoideum

The powerful genome editing tool Streptococcus pyogenes Cas9 (SpCas9) requires the trinucleotide NGG as a protospacer adjacent motif (PAM). The PAM requirement is limitation for precise genome editing such as single amino-acid substitutions and knock-ins at specific genomic loci since it occurs in n...

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Detalles Bibliográficos
Autores principales: Asano, Yuu, Yamashita, Kensuke, Hasegawa, Aoi, Ogasawara, Takanori, Iriki, Hoshie, Muramoto, Tetsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159936/
https://www.ncbi.nlm.nih.gov/pubmed/34045481
http://dx.doi.org/10.1038/s41598-021-89546-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159936/
https://www.ncbi.nlm.nih.gov/pubmed/34045481
http://dx.doi.org/10.1038/s41598-021-89546-0

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