Cargando…

Interrogating surface versus intracellular transmembrane receptor populations using cell-impermeable SNAP-tag substrates

Employing self-labelling protein tags for the attachment of fluorescent dyes has become a routine and powerful technique in optical microscopy to visualize and track fused proteins. However, membrane permeability of the dyes and the associated background signals can interfere with the analysis of ex...

Descripción completa

Detalles Bibliográficos
Autores principales: Poc, Pascal, Gutzeit, Vanessa A., Ast, Julia, Lee, Joon, Jones, Ben J., D'Este, Elisa, Mathes, Bettina, Lehmann, Martin, Hodson, David J., Levitz, Joshua, Broichhagen, Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163392/
https://www.ncbi.nlm.nih.gov/pubmed/34123074
http://dx.doi.org/10.1039/d0sc02794d