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Interrogating surface versus intracellular transmembrane receptor populations using cell-impermeable SNAP-tag substrates
Employing self-labelling protein tags for the attachment of fluorescent dyes has become a routine and powerful technique in optical microscopy to visualize and track fused proteins. However, membrane permeability of the dyes and the associated background signals can interfere with the analysis of ex...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The Royal Society of Chemistry
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163392/ https://www.ncbi.nlm.nih.gov/pubmed/34123074 http://dx.doi.org/10.1039/d0sc02794d |