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The noncovalent dimerization of a G-quadruplex/hemin DNAzyme improves its biocatalytic properties

While many protein enzymes exert their functions through multimerization, which improves both selectivity and activity, this has not yet been demonstrated for other naturally occurring catalysts. Here, we report a multimerization effect applied to catalytic DNAs (or DNAzymes) and demonstrate that th...

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Autores principales: Cheng, Yu, Cheng, Mingpan, Hao, Jingya, Jia, Guoqing, Monchaud, David, Li, Can
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163442/
https://www.ncbi.nlm.nih.gov/pubmed/34123138
http://dx.doi.org/10.1039/d0sc02907f
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author Cheng, Yu
Cheng, Mingpan
Hao, Jingya
Jia, Guoqing
Monchaud, David
Li, Can
author_facet Cheng, Yu
Cheng, Mingpan
Hao, Jingya
Jia, Guoqing
Monchaud, David
Li, Can
author_sort Cheng, Yu
collection PubMed
description While many protein enzymes exert their functions through multimerization, which improves both selectivity and activity, this has not yet been demonstrated for other naturally occurring catalysts. Here, we report a multimerization effect applied to catalytic DNAs (or DNAzymes) and demonstrate that the enzymatic efficiency of G-quadruplexes (GQs) in interaction with the hemin cofactor is remarkably enhanced by homodimerization. The resulting non-covalent dimeric GQ–DNAzyme system provides hemin with a structurally defined active site in which both the cofactor (hemin) and the oxidant (H(2)O(2)) are activated. This new biocatalytic system efficiently performs peroxidase- and peroxygenase-type biotransformations of a broad range of substrates, thus providing new perspectives for biotechnological application of GQs.
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spelling pubmed-81634422021-06-11 The noncovalent dimerization of a G-quadruplex/hemin DNAzyme improves its biocatalytic properties Cheng, Yu Cheng, Mingpan Hao, Jingya Jia, Guoqing Monchaud, David Li, Can Chem Sci Chemistry While many protein enzymes exert their functions through multimerization, which improves both selectivity and activity, this has not yet been demonstrated for other naturally occurring catalysts. Here, we report a multimerization effect applied to catalytic DNAs (or DNAzymes) and demonstrate that the enzymatic efficiency of G-quadruplexes (GQs) in interaction with the hemin cofactor is remarkably enhanced by homodimerization. The resulting non-covalent dimeric GQ–DNAzyme system provides hemin with a structurally defined active site in which both the cofactor (hemin) and the oxidant (H(2)O(2)) are activated. This new biocatalytic system efficiently performs peroxidase- and peroxygenase-type biotransformations of a broad range of substrates, thus providing new perspectives for biotechnological application of GQs. The Royal Society of Chemistry 2020-08-12 /pmc/articles/PMC8163442/ /pubmed/34123138 http://dx.doi.org/10.1039/d0sc02907f Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Cheng, Yu
Cheng, Mingpan
Hao, Jingya
Jia, Guoqing
Monchaud, David
Li, Can
The noncovalent dimerization of a G-quadruplex/hemin DNAzyme improves its biocatalytic properties
title The noncovalent dimerization of a G-quadruplex/hemin DNAzyme improves its biocatalytic properties
title_full The noncovalent dimerization of a G-quadruplex/hemin DNAzyme improves its biocatalytic properties
title_fullStr The noncovalent dimerization of a G-quadruplex/hemin DNAzyme improves its biocatalytic properties
title_full_unstemmed The noncovalent dimerization of a G-quadruplex/hemin DNAzyme improves its biocatalytic properties
title_short The noncovalent dimerization of a G-quadruplex/hemin DNAzyme improves its biocatalytic properties
title_sort noncovalent dimerization of a g-quadruplex/hemin dnazyme improves its biocatalytic properties
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163442/
https://www.ncbi.nlm.nih.gov/pubmed/34123138
http://dx.doi.org/10.1039/d0sc02907f
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