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An optimized protocol for isolation of S-nitrosylated proteins from C. elegans

Post-translational modification by S-nitrosylation regulates numerous cellular functions and impacts most proteins across phylogeny. We describe a protocol for isolating S-nitrosylated proteins (SNO-proteins) from C. elegans, suitable for assessing SNO levels of individual proteins and of the global...

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Detalles Bibliográficos
Autores principales: Seth, Puneet, Premont, Richard T., Stamler, Jonathan S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164088/
https://www.ncbi.nlm.nih.gov/pubmed/34095861
http://dx.doi.org/10.1016/j.xpro.2021.100547