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An optimized protocol for isolation of S-nitrosylated proteins from C. elegans
Post-translational modification by S-nitrosylation regulates numerous cellular functions and impacts most proteins across phylogeny. We describe a protocol for isolating S-nitrosylated proteins (SNO-proteins) from C. elegans, suitable for assessing SNO levels of individual proteins and of the global...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164088/ https://www.ncbi.nlm.nih.gov/pubmed/34095861 http://dx.doi.org/10.1016/j.xpro.2021.100547 |