An optimized protocol for isolation of S-nitrosylated proteins from C. elegans

Post-translational modification by S-nitrosylation regulates numerous cellular functions and impacts most proteins across phylogeny. We describe a protocol for isolating S-nitrosylated proteins (SNO-proteins) from C. elegans, suitable for assessing SNO levels of individual proteins and of the global...

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Detalles Bibliográficos
Autores principales: Seth, Puneet, Premont, Richard T., Stamler, Jonathan S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164088/
https://www.ncbi.nlm.nih.gov/pubmed/34095861
http://dx.doi.org/10.1016/j.xpro.2021.100547
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author Seth, Puneet
Premont, Richard T.
Stamler, Jonathan S.
author_facet Seth, Puneet
Premont, Richard T.
Stamler, Jonathan S.
author_sort Seth, Puneet
collection PubMed
description Post-translational modification by S-nitrosylation regulates numerous cellular functions and impacts most proteins across phylogeny. We describe a protocol for isolating S-nitrosylated proteins (SNO-proteins) from C. elegans, suitable for assessing SNO levels of individual proteins and of the global proteome. This protocol features efficient nematode lysis and SNO capture, while protection of SNO proteins from degradation is the major challenge. This protocol can be adapted to mammalian tissues. For complete information on the generation and use of this protocol, please refer to Seth et al. (2019).
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spelling pubmed-81640882021-06-04 An optimized protocol for isolation of S-nitrosylated proteins from C. elegans Seth, Puneet Premont, Richard T. Stamler, Jonathan S. STAR Protoc Protocol Post-translational modification by S-nitrosylation regulates numerous cellular functions and impacts most proteins across phylogeny. We describe a protocol for isolating S-nitrosylated proteins (SNO-proteins) from C. elegans, suitable for assessing SNO levels of individual proteins and of the global proteome. This protocol features efficient nematode lysis and SNO capture, while protection of SNO proteins from degradation is the major challenge. This protocol can be adapted to mammalian tissues. For complete information on the generation and use of this protocol, please refer to Seth et al. (2019). Elsevier 2021-05-19 /pmc/articles/PMC8164088/ /pubmed/34095861 http://dx.doi.org/10.1016/j.xpro.2021.100547 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Seth, Puneet
Premont, Richard T.
Stamler, Jonathan S.
An optimized protocol for isolation of S-nitrosylated proteins from C. elegans
title An optimized protocol for isolation of S-nitrosylated proteins from C. elegans
title_full An optimized protocol for isolation of S-nitrosylated proteins from C. elegans
title_fullStr An optimized protocol for isolation of S-nitrosylated proteins from C. elegans
title_full_unstemmed An optimized protocol for isolation of S-nitrosylated proteins from C. elegans
title_short An optimized protocol for isolation of S-nitrosylated proteins from C. elegans
title_sort optimized protocol for isolation of s-nitrosylated proteins from c. elegans
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164088/
https://www.ncbi.nlm.nih.gov/pubmed/34095861
http://dx.doi.org/10.1016/j.xpro.2021.100547
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