Protocol for genome-scale CRISPR screening in engineered lineage reporter hPSCs to study cell fate determination

PAX6 is a key determinant of human neuroectoderm cell fate. Here, we describe a protocol for genome-scale CRISPR screening for use in genetically engineered human pluripotent stem cells (hPSCs). Using the germ layer reporter PAX6 and an inducible CRISPR/Cas9 knockout system, we describe how to ident...

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Detalles Bibliográficos
Autores principales: Zhou, Zhongshu, Ma, Lin, Zhang, Xiaoqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164093/
https://www.ncbi.nlm.nih.gov/pubmed/34095862
http://dx.doi.org/10.1016/j.xpro.2021.100548

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164093/
https://www.ncbi.nlm.nih.gov/pubmed/34095862
http://dx.doi.org/10.1016/j.xpro.2021.100548

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