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Protocol for genome-scale CRISPR screening in engineered lineage reporter hPSCs to study cell fate determination

PAX6 is a key determinant of human neuroectoderm cell fate. Here, we describe a protocol for genome-scale CRISPR screening for use in genetically engineered human pluripotent stem cells (hPSCs). Using the germ layer reporter PAX6 and an inducible CRISPR/Cas9 knockout system, we describe how to ident...

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Detalles Bibliográficos
Autores principales:	Zhou, Zhongshu, Ma, Lin, Zhang, Xiaoqing
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164093/ https://www.ncbi.nlm.nih.gov/pubmed/34095862 http://dx.doi.org/10.1016/j.xpro.2021.100548

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