One-step CRISPR-Cas9 protocol for the generation of plug & play conditional knockouts in Drosophila melanogaster

This one-step method generates, for any locus, a conditional knockout allele in Drosophila. The allele carries a bright fluorescent marker for easy screening and an attP landing site for subsequent genetic manipulations. After removing the selectable marker with Cre, the attP site can be used to ins...

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Detalles Bibliográficos
Autores principales: Yu, Joyce J.S., Vincent, Jean-Paul, McGough, Ian J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165570/
https://www.ncbi.nlm.nih.gov/pubmed/34095868
http://dx.doi.org/10.1016/j.xpro.2021.100560

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165570/
https://www.ncbi.nlm.nih.gov/pubmed/34095868
http://dx.doi.org/10.1016/j.xpro.2021.100560

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