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One-step CRISPR-Cas9 protocol for the generation of plug & play conditional knockouts in Drosophila melanogaster
This one-step method generates, for any locus, a conditional knockout allele in Drosophila. The allele carries a bright fluorescent marker for easy screening and an attP landing site for subsequent genetic manipulations. After removing the selectable marker with Cre, the attP site can be used to ins...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165570/ https://www.ncbi.nlm.nih.gov/pubmed/34095868 http://dx.doi.org/10.1016/j.xpro.2021.100560 |