A phenotypically supervised single-cell analysis protocol to study within-cell-type heterogeneity of cultured mammalian cells

Here, we describe a protocol combining functional metrics with genomic data to elucidate drivers of within-cell-type heterogeneity via the phenotype-to-genotype link. This technique involves using fluorescence tagging to label and isolate cells grown in 3D culture, enabling high-throughput enrichmen...

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Detalles Bibliográficos
Autores principales: Chen, Kevin, Ozturk, Kivilcim, Liefeld, Ted, Reich, Michael, Mesirov, Jill P., Carter, Hannah, Fraley, Stephanie I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165572/
https://www.ncbi.nlm.nih.gov/pubmed/34095869
http://dx.doi.org/10.1016/j.xpro.2021.100561

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165572/
https://www.ncbi.nlm.nih.gov/pubmed/34095869
http://dx.doi.org/10.1016/j.xpro.2021.100561

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