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Combining Mass Spectrometry-Based Phosphoproteomics with a Network-Based Approach to Reveal FLT3-Dependent Mechanisms of Chemoresistance

FLT3 mutations are the most frequently identified genetic alterations in acute myeloid leukemia (AML) and are associated with poor clinical outcome, relapse and chemotherapeutic resistance. Elucidating the molecular mechanisms underlying FLT3-dependent pathogenesis and drug resistance is a crucial g...

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Autores principales: Pugliese, Giusj Monia, Latini, Sara, Massacci, Giorgia, Perfetto, Livia, Sacco, Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167576/
https://www.ncbi.nlm.nih.gov/pubmed/33925552
http://dx.doi.org/10.3390/proteomes9020019
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author Pugliese, Giusj Monia
Latini, Sara
Massacci, Giorgia
Perfetto, Livia
Sacco, Francesca
author_facet Pugliese, Giusj Monia
Latini, Sara
Massacci, Giorgia
Perfetto, Livia
Sacco, Francesca
author_sort Pugliese, Giusj Monia
collection PubMed
description FLT3 mutations are the most frequently identified genetic alterations in acute myeloid leukemia (AML) and are associated with poor clinical outcome, relapse and chemotherapeutic resistance. Elucidating the molecular mechanisms underlying FLT3-dependent pathogenesis and drug resistance is a crucial goal of biomedical research. Given the complexity and intricacy of protein signaling networks, deciphering the molecular basis of FLT3-driven drug resistance requires a systems approach. Here we discuss how the recent advances in mass spectrometry (MS)-based (phospho) proteomics and multiparametric analysis accompanied by emerging computational approaches offer a platform to obtain and systematically analyze cell-specific signaling networks and to identify new potential therapeutic targets.
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spelling pubmed-81675762021-06-02 Combining Mass Spectrometry-Based Phosphoproteomics with a Network-Based Approach to Reveal FLT3-Dependent Mechanisms of Chemoresistance Pugliese, Giusj Monia Latini, Sara Massacci, Giorgia Perfetto, Livia Sacco, Francesca Proteomes Review FLT3 mutations are the most frequently identified genetic alterations in acute myeloid leukemia (AML) and are associated with poor clinical outcome, relapse and chemotherapeutic resistance. Elucidating the molecular mechanisms underlying FLT3-dependent pathogenesis and drug resistance is a crucial goal of biomedical research. Given the complexity and intricacy of protein signaling networks, deciphering the molecular basis of FLT3-driven drug resistance requires a systems approach. Here we discuss how the recent advances in mass spectrometry (MS)-based (phospho) proteomics and multiparametric analysis accompanied by emerging computational approaches offer a platform to obtain and systematically analyze cell-specific signaling networks and to identify new potential therapeutic targets. MDPI 2021-04-27 /pmc/articles/PMC8167576/ /pubmed/33925552 http://dx.doi.org/10.3390/proteomes9020019 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Pugliese, Giusj Monia
Latini, Sara
Massacci, Giorgia
Perfetto, Livia
Sacco, Francesca
Combining Mass Spectrometry-Based Phosphoproteomics with a Network-Based Approach to Reveal FLT3-Dependent Mechanisms of Chemoresistance
title Combining Mass Spectrometry-Based Phosphoproteomics with a Network-Based Approach to Reveal FLT3-Dependent Mechanisms of Chemoresistance
title_full Combining Mass Spectrometry-Based Phosphoproteomics with a Network-Based Approach to Reveal FLT3-Dependent Mechanisms of Chemoresistance
title_fullStr Combining Mass Spectrometry-Based Phosphoproteomics with a Network-Based Approach to Reveal FLT3-Dependent Mechanisms of Chemoresistance
title_full_unstemmed Combining Mass Spectrometry-Based Phosphoproteomics with a Network-Based Approach to Reveal FLT3-Dependent Mechanisms of Chemoresistance
title_short Combining Mass Spectrometry-Based Phosphoproteomics with a Network-Based Approach to Reveal FLT3-Dependent Mechanisms of Chemoresistance
title_sort combining mass spectrometry-based phosphoproteomics with a network-based approach to reveal flt3-dependent mechanisms of chemoresistance
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167576/
https://www.ncbi.nlm.nih.gov/pubmed/33925552
http://dx.doi.org/10.3390/proteomes9020019
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