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Inner Nuclear Membrane Asi Ubiquitin Ligase Catalytic Subunits Asi1p and Asi3p, but not Asi2p, confer resistance to aminoglycoside hygromycin B in Saccharomyces cerevisiae

The heterotrimeric Asi ubiquitin ligase (encoded by ASI1, ASI2, and ASI3) mediates protein degradation in the inner nuclear membrane in Saccharomyces cerevisiae. Asi1p and Asi3p possess catalytic domains, while Asi2p functions as an adaptor for a subset of Asi substrates. We hypothesized the Asi com...

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Autores principales: Woodruff, Kelsey A, Richards, Kyle A, Evans, Melissa D, Scott, Abigail R, Voas, Brian M, Irelan, Courtney Broshar, Olesen, James B, Smaldino, Philip J, Rubenstein, Eric M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170509/
https://www.ncbi.nlm.nih.gov/pubmed/34095778
http://dx.doi.org/10.17912/micropub.biology.000403
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author Woodruff, Kelsey A
Richards, Kyle A
Evans, Melissa D
Scott, Abigail R
Voas, Brian M
Irelan, Courtney Broshar
Olesen, James B
Smaldino, Philip J
Rubenstein, Eric M
author_facet Woodruff, Kelsey A
Richards, Kyle A
Evans, Melissa D
Scott, Abigail R
Voas, Brian M
Irelan, Courtney Broshar
Olesen, James B
Smaldino, Philip J
Rubenstein, Eric M
author_sort Woodruff, Kelsey A
collection PubMed
description The heterotrimeric Asi ubiquitin ligase (encoded by ASI1, ASI2, and ASI3) mediates protein degradation in the inner nuclear membrane in Saccharomyces cerevisiae. Asi1p and Asi3p possess catalytic domains, while Asi2p functions as an adaptor for a subset of Asi substrates. We hypothesized the Asi complex is an important mediator of protein quality control, and we predicted that Asi would be required for optimal growth in conditions associated with elevated abundance of aberrant proteins. Loss of Asi1p or Asi3p, but not Asi2p, sensitized yeast to hygromycin B, which promotes translational infidelity by distorting the ribosome A site. Surprisingly, loss of quality control ubiquitin ligase Hul5p did not sensitize yeast to hygromycin B. Our results are consistent with a prominent role for an Asi subcomplex that includes Asi1p and Asi3p (but not Asi2p) in protein quality control.
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spelling pubmed-81705092021-06-04 Inner Nuclear Membrane Asi Ubiquitin Ligase Catalytic Subunits Asi1p and Asi3p, but not Asi2p, confer resistance to aminoglycoside hygromycin B in Saccharomyces cerevisiae Woodruff, Kelsey A Richards, Kyle A Evans, Melissa D Scott, Abigail R Voas, Brian M Irelan, Courtney Broshar Olesen, James B Smaldino, Philip J Rubenstein, Eric M MicroPubl Biol New Finding The heterotrimeric Asi ubiquitin ligase (encoded by ASI1, ASI2, and ASI3) mediates protein degradation in the inner nuclear membrane in Saccharomyces cerevisiae. Asi1p and Asi3p possess catalytic domains, while Asi2p functions as an adaptor for a subset of Asi substrates. We hypothesized the Asi complex is an important mediator of protein quality control, and we predicted that Asi would be required for optimal growth in conditions associated with elevated abundance of aberrant proteins. Loss of Asi1p or Asi3p, but not Asi2p, sensitized yeast to hygromycin B, which promotes translational infidelity by distorting the ribosome A site. Surprisingly, loss of quality control ubiquitin ligase Hul5p did not sensitize yeast to hygromycin B. Our results are consistent with a prominent role for an Asi subcomplex that includes Asi1p and Asi3p (but not Asi2p) in protein quality control. Caltech Library 2021-06-01 /pmc/articles/PMC8170509/ /pubmed/34095778 http://dx.doi.org/10.17912/micropub.biology.000403 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Woodruff, Kelsey A
Richards, Kyle A
Evans, Melissa D
Scott, Abigail R
Voas, Brian M
Irelan, Courtney Broshar
Olesen, James B
Smaldino, Philip J
Rubenstein, Eric M
Inner Nuclear Membrane Asi Ubiquitin Ligase Catalytic Subunits Asi1p and Asi3p, but not Asi2p, confer resistance to aminoglycoside hygromycin B in Saccharomyces cerevisiae
title Inner Nuclear Membrane Asi Ubiquitin Ligase Catalytic Subunits Asi1p and Asi3p, but not Asi2p, confer resistance to aminoglycoside hygromycin B in Saccharomyces cerevisiae
title_full Inner Nuclear Membrane Asi Ubiquitin Ligase Catalytic Subunits Asi1p and Asi3p, but not Asi2p, confer resistance to aminoglycoside hygromycin B in Saccharomyces cerevisiae
title_fullStr Inner Nuclear Membrane Asi Ubiquitin Ligase Catalytic Subunits Asi1p and Asi3p, but not Asi2p, confer resistance to aminoglycoside hygromycin B in Saccharomyces cerevisiae
title_full_unstemmed Inner Nuclear Membrane Asi Ubiquitin Ligase Catalytic Subunits Asi1p and Asi3p, but not Asi2p, confer resistance to aminoglycoside hygromycin B in Saccharomyces cerevisiae
title_short Inner Nuclear Membrane Asi Ubiquitin Ligase Catalytic Subunits Asi1p and Asi3p, but not Asi2p, confer resistance to aminoglycoside hygromycin B in Saccharomyces cerevisiae
title_sort inner nuclear membrane asi ubiquitin ligase catalytic subunits asi1p and asi3p, but not asi2p, confer resistance to aminoglycoside hygromycin b in saccharomyces cerevisiae
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170509/
https://www.ncbi.nlm.nih.gov/pubmed/34095778
http://dx.doi.org/10.17912/micropub.biology.000403
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