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Brain pharmacokinetics of two BBB penetrating bispecific antibodies of different size
BACKGROUND: Transferrin receptor (TfR1) mediated enhanced brain delivery of antibodies have been studied extensively in preclinical settings. However, the brain pharmacokinetics, i.e. brain entry, distribution and elimination are still not fully understood for this class of antibodies. The overall a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170802/ https://www.ncbi.nlm.nih.gov/pubmed/34078410 http://dx.doi.org/10.1186/s12987-021-00257-0 |
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author | Faresjö, Rebecca Bonvicini, Gillian Fang, Xiaotian T. Aguilar, Ximena Sehlin, Dag Syvänen, Stina |
author_facet | Faresjö, Rebecca Bonvicini, Gillian Fang, Xiaotian T. Aguilar, Ximena Sehlin, Dag Syvänen, Stina |
author_sort | Faresjö, Rebecca |
collection | PubMed |
description | BACKGROUND: Transferrin receptor (TfR1) mediated enhanced brain delivery of antibodies have been studied extensively in preclinical settings. However, the brain pharmacokinetics, i.e. brain entry, distribution and elimination are still not fully understood for this class of antibodies. The overall aim of the study was to compare the brain pharmacokinetics of two BBB-penetrating bispecific antibodies of different size (210 vs 58 kDa). Specifically, we wanted to investigate if the faster systemic clearance of the smaller non-IgG antibody di-scFv3D6-8D3, in comparison with the IgG-based bispecific antibody mAb3D6-scFv8D3, was also reflected in the brain. METHODS: Wild-type (C57/Bl6) mice were injected with (125)I-iodinated ([(125)I]) mAb3D6-scFv8D3 (n = 46) or [(125)I]di-scFv3D6-8D3 (n = 32) and euthanized 2, 4, 6, 8, 10, 12, 16, or 24 h post injection. Ex vivo radioactivity in whole blood, peripheral organs and brain was measured by γ-counting. Ex vivo autoradiography and nuclear track emulsion were performed on brain sections to investigate brain and parenchymal distribution. Capillary depletion was carried out at 2, 6, and 24 h after injection of [(125)I]mAb3D6-scFv8D3 (n = 12) or [(125)I]di-scFv3D6-8D3 (n = 12), to estimate the relative levels of radiolabelled antibody in brain capillaries versus brain parenchyma. In vitro binding kinetics for [(125)I]mAb3D6-scFv8D3 or [(125)I]di-scFv3D6-8D3 to murine TfR were determined by LigandTracer. RESULTS: [(125)I]di-scFv3D6-8D3 showed faster elimination from blood, lower brain C(max), and T(max), a larger parenchymal-to-capillary concentration ratio, and a net elimination from brain at an earlier time point after injection compared with the larger [(125)I]mAb3D6-scFv8D3. However, the elimination rate from brain did not differ between the antibodies. The study also indicated that [(125)I]di-scFv3D6-8D3 displayed lower avidity than [(125)I]mAb3D6-scFv8D3 towards TfR1 in vitro and potentially in vivo, at least at the BBB. CONCLUSION: A smaller size and lower TfR1 avidity are likely important for fast parenchymal delivery, while elimination of brain-associated bispecific antibodies may not be dependent on these characteristics. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12987-021-00257-0. |
format | Online Article Text |
id | pubmed-8170802 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-81708022021-06-02 Brain pharmacokinetics of two BBB penetrating bispecific antibodies of different size Faresjö, Rebecca Bonvicini, Gillian Fang, Xiaotian T. Aguilar, Ximena Sehlin, Dag Syvänen, Stina Fluids Barriers CNS Research BACKGROUND: Transferrin receptor (TfR1) mediated enhanced brain delivery of antibodies have been studied extensively in preclinical settings. However, the brain pharmacokinetics, i.e. brain entry, distribution and elimination are still not fully understood for this class of antibodies. The overall aim of the study was to compare the brain pharmacokinetics of two BBB-penetrating bispecific antibodies of different size (210 vs 58 kDa). Specifically, we wanted to investigate if the faster systemic clearance of the smaller non-IgG antibody di-scFv3D6-8D3, in comparison with the IgG-based bispecific antibody mAb3D6-scFv8D3, was also reflected in the brain. METHODS: Wild-type (C57/Bl6) mice were injected with (125)I-iodinated ([(125)I]) mAb3D6-scFv8D3 (n = 46) or [(125)I]di-scFv3D6-8D3 (n = 32) and euthanized 2, 4, 6, 8, 10, 12, 16, or 24 h post injection. Ex vivo radioactivity in whole blood, peripheral organs and brain was measured by γ-counting. Ex vivo autoradiography and nuclear track emulsion were performed on brain sections to investigate brain and parenchymal distribution. Capillary depletion was carried out at 2, 6, and 24 h after injection of [(125)I]mAb3D6-scFv8D3 (n = 12) or [(125)I]di-scFv3D6-8D3 (n = 12), to estimate the relative levels of radiolabelled antibody in brain capillaries versus brain parenchyma. In vitro binding kinetics for [(125)I]mAb3D6-scFv8D3 or [(125)I]di-scFv3D6-8D3 to murine TfR were determined by LigandTracer. RESULTS: [(125)I]di-scFv3D6-8D3 showed faster elimination from blood, lower brain C(max), and T(max), a larger parenchymal-to-capillary concentration ratio, and a net elimination from brain at an earlier time point after injection compared with the larger [(125)I]mAb3D6-scFv8D3. However, the elimination rate from brain did not differ between the antibodies. The study also indicated that [(125)I]di-scFv3D6-8D3 displayed lower avidity than [(125)I]mAb3D6-scFv8D3 towards TfR1 in vitro and potentially in vivo, at least at the BBB. CONCLUSION: A smaller size and lower TfR1 avidity are likely important for fast parenchymal delivery, while elimination of brain-associated bispecific antibodies may not be dependent on these characteristics. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12987-021-00257-0. BioMed Central 2021-06-02 /pmc/articles/PMC8170802/ /pubmed/34078410 http://dx.doi.org/10.1186/s12987-021-00257-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Faresjö, Rebecca Bonvicini, Gillian Fang, Xiaotian T. Aguilar, Ximena Sehlin, Dag Syvänen, Stina Brain pharmacokinetics of two BBB penetrating bispecific antibodies of different size |
title | Brain pharmacokinetics of two BBB penetrating bispecific antibodies of different size |
title_full | Brain pharmacokinetics of two BBB penetrating bispecific antibodies of different size |
title_fullStr | Brain pharmacokinetics of two BBB penetrating bispecific antibodies of different size |
title_full_unstemmed | Brain pharmacokinetics of two BBB penetrating bispecific antibodies of different size |
title_short | Brain pharmacokinetics of two BBB penetrating bispecific antibodies of different size |
title_sort | brain pharmacokinetics of two bbb penetrating bispecific antibodies of different size |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170802/ https://www.ncbi.nlm.nih.gov/pubmed/34078410 http://dx.doi.org/10.1186/s12987-021-00257-0 |
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