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HSPCs display within-family homogeneity in differentiation and proliferation despite population heterogeneity

High-throughput single-cell methods have uncovered substantial heterogeneity in the pool of hematopoietic stem and progenitor cells (HSPCs), but how much instruction is inherited by offspring from their heterogeneous ancestors remains unanswered. Using a method that enables simultaneous determinatio...

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Autores principales: Tak, Tamar, Prevedello, Giulio, Simon, Gaël, Paillon, Noémie, Benlabiod, Camélia, Marty, Caroline, Plo, Isabelle, Duffy, Ken R, Perié, Leïla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175087/
https://www.ncbi.nlm.nih.gov/pubmed/34002698
http://dx.doi.org/10.7554/eLife.60624
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author Tak, Tamar
Prevedello, Giulio
Simon, Gaël
Paillon, Noémie
Benlabiod, Camélia
Marty, Caroline
Plo, Isabelle
Duffy, Ken R
Perié, Leïla
author_facet Tak, Tamar
Prevedello, Giulio
Simon, Gaël
Paillon, Noémie
Benlabiod, Camélia
Marty, Caroline
Plo, Isabelle
Duffy, Ken R
Perié, Leïla
author_sort Tak, Tamar
collection PubMed
description High-throughput single-cell methods have uncovered substantial heterogeneity in the pool of hematopoietic stem and progenitor cells (HSPCs), but how much instruction is inherited by offspring from their heterogeneous ancestors remains unanswered. Using a method that enables simultaneous determination of common ancestor, division number, and differentiation status of a large collection of single cells, our data revealed that murine cells that derived from a common ancestor had significant similarities in their division progression and differentiation outcomes. Although each family diversifies, the overall collection of cell types observed is composed of homogeneous families. Heterogeneity between families could be explained, in part, by differences in ancestral expression of cell surface markers. Our analyses demonstrate that fate decisions of cells are largely inherited from ancestor cells, indicating the importance of common ancestor effects. These results may have ramifications for bone marrow transplantation and leukemia, where substantial heterogeneity in HSPC behavior is observed.
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spelling pubmed-81750872021-06-04 HSPCs display within-family homogeneity in differentiation and proliferation despite population heterogeneity Tak, Tamar Prevedello, Giulio Simon, Gaël Paillon, Noémie Benlabiod, Camélia Marty, Caroline Plo, Isabelle Duffy, Ken R Perié, Leïla eLife Stem Cells and Regenerative Medicine High-throughput single-cell methods have uncovered substantial heterogeneity in the pool of hematopoietic stem and progenitor cells (HSPCs), but how much instruction is inherited by offspring from their heterogeneous ancestors remains unanswered. Using a method that enables simultaneous determination of common ancestor, division number, and differentiation status of a large collection of single cells, our data revealed that murine cells that derived from a common ancestor had significant similarities in their division progression and differentiation outcomes. Although each family diversifies, the overall collection of cell types observed is composed of homogeneous families. Heterogeneity between families could be explained, in part, by differences in ancestral expression of cell surface markers. Our analyses demonstrate that fate decisions of cells are largely inherited from ancestor cells, indicating the importance of common ancestor effects. These results may have ramifications for bone marrow transplantation and leukemia, where substantial heterogeneity in HSPC behavior is observed. eLife Sciences Publications, Ltd 2021-05-18 /pmc/articles/PMC8175087/ /pubmed/34002698 http://dx.doi.org/10.7554/eLife.60624 Text en © 2021, Tak et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Stem Cells and Regenerative Medicine
Tak, Tamar
Prevedello, Giulio
Simon, Gaël
Paillon, Noémie
Benlabiod, Camélia
Marty, Caroline
Plo, Isabelle
Duffy, Ken R
Perié, Leïla
HSPCs display within-family homogeneity in differentiation and proliferation despite population heterogeneity
title HSPCs display within-family homogeneity in differentiation and proliferation despite population heterogeneity
title_full HSPCs display within-family homogeneity in differentiation and proliferation despite population heterogeneity
title_fullStr HSPCs display within-family homogeneity in differentiation and proliferation despite population heterogeneity
title_full_unstemmed HSPCs display within-family homogeneity in differentiation and proliferation despite population heterogeneity
title_short HSPCs display within-family homogeneity in differentiation and proliferation despite population heterogeneity
title_sort hspcs display within-family homogeneity in differentiation and proliferation despite population heterogeneity
topic Stem Cells and Regenerative Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175087/
https://www.ncbi.nlm.nih.gov/pubmed/34002698
http://dx.doi.org/10.7554/eLife.60624
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