Numerical optimization of microfluidic vortex shedding for genome editing T cells with Cas9

Microfluidic vortex shedding (µVS) can rapidly deliver mRNA to T cells with high yield and minimal perturbation of the cell state. The mechanistic underpinning of µVS intracellular delivery remains undefined and µVS-Cas9 genome editing requires further studies. Herein, we evaluated a series of µVS d...

Descripción completa

Detalles Bibliográficos
Autores principales: Jarrell, Justin A., Sytsma, Brandon J., Wilson, Leah H., Pan, Fong L., Lau, Katherine H. W. J., Kirby, Giles T. S., Lievano, Adrian A., Pawell, Ryan S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175688/
https://www.ncbi.nlm.nih.gov/pubmed/34083685
http://dx.doi.org/10.1038/s41598-021-91307-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175688/
https://www.ncbi.nlm.nih.gov/pubmed/34083685
http://dx.doi.org/10.1038/s41598-021-91307-y

Ejemplares similares