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An in vivo selection system with tightly regulated gene expression enables directed evolution of highly efficient enzymes
In vivo selection systems are powerful tools for directed evolution of enzymes. The selection pressure of the systems can be tuned by regulating the expression levels of the catalysts. In this work, we engineered a selection system for laboratory evolution of highly active enzymes by incorporating a...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175713/ https://www.ncbi.nlm.nih.gov/pubmed/34083677 http://dx.doi.org/10.1038/s41598-021-91204-4 |