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An in vivo selection system with tightly regulated gene expression enables directed evolution of highly efficient enzymes

In vivo selection systems are powerful tools for directed evolution of enzymes. The selection pressure of the systems can be tuned by regulating the expression levels of the catalysts. In this work, we engineered a selection system for laboratory evolution of highly active enzymes by incorporating a...

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Detalles Bibliográficos
Autores principales: Nearmnala, Parinthon, Thanaburakorn, Manutsawee, Panbangred, Watanalai, Chaiyen, Pimchai, Hongdilokkul, Narupat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175713/
https://www.ncbi.nlm.nih.gov/pubmed/34083677
http://dx.doi.org/10.1038/s41598-021-91204-4

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