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LIN28A inhibits DUSP family phosphatases and activates MAPK signaling pathway to maintain pluripotency in porcine induced pluripotent stem cells
LIN28A, an RNA-binding protein, plays an important role in porcine induced pluripotent stem cells (piPSCs). However, the molecular mechanism underlying the function of LIN28A in the maintenance of pluripotency in piPSCs remains unclear. Here, we explored the function of LIN28A in piPSCs based on its...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Science Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175949/ https://www.ncbi.nlm.nih.gov/pubmed/33998185 http://dx.doi.org/10.24272/j.issn.2095-8137.2020.375 |
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author | Wu, Xiao-Long Zhu, Zhen-Shuo Xiao, Xia Zhou, Zhe Yu, Shuai Shen, Qiao-Yan Zhang, Ju-Qing Yue, Wei Zhang, Rui He, Xin Peng, Sha Zhang, Shi-Qiang Li, Na Liao, Ming-Zhi Hua, Jin-Lian |
author_facet | Wu, Xiao-Long Zhu, Zhen-Shuo Xiao, Xia Zhou, Zhe Yu, Shuai Shen, Qiao-Yan Zhang, Ju-Qing Yue, Wei Zhang, Rui He, Xin Peng, Sha Zhang, Shi-Qiang Li, Na Liao, Ming-Zhi Hua, Jin-Lian |
author_sort | Wu, Xiao-Long |
collection | PubMed |
description | LIN28A, an RNA-binding protein, plays an important role in porcine induced pluripotent stem cells (piPSCs). However, the molecular mechanism underlying the function of LIN28A in the maintenance of pluripotency in piPSCs remains unclear. Here, we explored the function of LIN28A in piPSCs based on its overexpression and knockdown. We performed total RNA sequencing (RNA-seq) of piPSCs and detected the expression levels of relevant genes by quantitative real-time polymerase chain reaction (qRT-PCR), western blot analysis, and immunofluorescence staining. Results indicated that piPSC proliferation ability decreased following LIN28A knockdown. Furthermore, when LIN28A expression in the shLIN28A2 group was lower (by 20%) than that in the negative control knockdown group (shNC), the pluripotency of piPSCs disappeared and they differentiated into neuroectoderm cells. Results also showed that LIN28A overexpression inhibited the expression of DUSP (dual-specificity phosphatases) family phosphatases and activated the mitogen-activated protein kinase (MAPK) signaling pathway. Thus, LIN28A appears to activate the MAPK signaling pathway to maintain the pluripotency and proliferation ability of piPSCs. Our study provides a new resource for exploring the functions of LIN28A in piPSCs. |
format | Online Article Text |
id | pubmed-8175949 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Science Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-81759492021-06-10 LIN28A inhibits DUSP family phosphatases and activates MAPK signaling pathway to maintain pluripotency in porcine induced pluripotent stem cells Wu, Xiao-Long Zhu, Zhen-Shuo Xiao, Xia Zhou, Zhe Yu, Shuai Shen, Qiao-Yan Zhang, Ju-Qing Yue, Wei Zhang, Rui He, Xin Peng, Sha Zhang, Shi-Qiang Li, Na Liao, Ming-Zhi Hua, Jin-Lian Zool Res Article LIN28A, an RNA-binding protein, plays an important role in porcine induced pluripotent stem cells (piPSCs). However, the molecular mechanism underlying the function of LIN28A in the maintenance of pluripotency in piPSCs remains unclear. Here, we explored the function of LIN28A in piPSCs based on its overexpression and knockdown. We performed total RNA sequencing (RNA-seq) of piPSCs and detected the expression levels of relevant genes by quantitative real-time polymerase chain reaction (qRT-PCR), western blot analysis, and immunofluorescence staining. Results indicated that piPSC proliferation ability decreased following LIN28A knockdown. Furthermore, when LIN28A expression in the shLIN28A2 group was lower (by 20%) than that in the negative control knockdown group (shNC), the pluripotency of piPSCs disappeared and they differentiated into neuroectoderm cells. Results also showed that LIN28A overexpression inhibited the expression of DUSP (dual-specificity phosphatases) family phosphatases and activated the mitogen-activated protein kinase (MAPK) signaling pathway. Thus, LIN28A appears to activate the MAPK signaling pathway to maintain the pluripotency and proliferation ability of piPSCs. Our study provides a new resource for exploring the functions of LIN28A in piPSCs. Science Press 2021-05-18 /pmc/articles/PMC8175949/ /pubmed/33998185 http://dx.doi.org/10.24272/j.issn.2095-8137.2020.375 Text en Editorial Office of Zoological Research, Kunming Institute of Zoology, Chinese Academy of Sciences https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Wu, Xiao-Long Zhu, Zhen-Shuo Xiao, Xia Zhou, Zhe Yu, Shuai Shen, Qiao-Yan Zhang, Ju-Qing Yue, Wei Zhang, Rui He, Xin Peng, Sha Zhang, Shi-Qiang Li, Na Liao, Ming-Zhi Hua, Jin-Lian LIN28A inhibits DUSP family phosphatases and activates MAPK signaling pathway to maintain pluripotency in porcine induced pluripotent stem cells |
title | LIN28A inhibits DUSP family phosphatases and activates MAPK signaling pathway to maintain pluripotency in porcine induced pluripotent stem cells
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title_full | LIN28A inhibits DUSP family phosphatases and activates MAPK signaling pathway to maintain pluripotency in porcine induced pluripotent stem cells
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title_fullStr | LIN28A inhibits DUSP family phosphatases and activates MAPK signaling pathway to maintain pluripotency in porcine induced pluripotent stem cells
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title_full_unstemmed | LIN28A inhibits DUSP family phosphatases and activates MAPK signaling pathway to maintain pluripotency in porcine induced pluripotent stem cells
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title_short | LIN28A inhibits DUSP family phosphatases and activates MAPK signaling pathway to maintain pluripotency in porcine induced pluripotent stem cells
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title_sort | lin28a inhibits dusp family phosphatases and activates mapk signaling pathway to maintain pluripotency in porcine induced pluripotent stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175949/ https://www.ncbi.nlm.nih.gov/pubmed/33998185 http://dx.doi.org/10.24272/j.issn.2095-8137.2020.375 |
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