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Sensitive intranuclear flow cytometric quantification of IRF4 protein in multiple myeloma and normal human hematopoietic cells

Interferon regulatory factor 4 (IRF4) is a transcription factor that regulates normal and malignant immune cell development and is implicated in multiple myeloma pathogenesis. This protocol describes the use of combined cell surface and intranuclear staining with fluorescent antibodies to measure IR...

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Detalles Bibliográficos
Autores principales: Vora, Ashni A., Mondala, Phoebe K., Costello, Caitlin, MacLeod, A. Robert, Crews, Leslie A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176358/
https://www.ncbi.nlm.nih.gov/pubmed/34136833
http://dx.doi.org/10.1016/j.xpro.2021.100565