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A ribonucleoprotein transfection strategy for CRISPR/Cas9‐mediated gene editing and single cell cloning in rainbow trout cells

BACKGROUND: The advent of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 technology marked the beginning of a new era in the field of molecular biology, allowing the efficient and precise creation of targeted mutations in the genome of every living cell. Since its discov...

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Detalles Bibliográficos
Autores principales: Zoppo, Marina, Okoniewski, Nicole, Pantelyushin, Stanislav, vom Berg, Johannes, Schirmer, Kristin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176604/
https://www.ncbi.nlm.nih.gov/pubmed/34082820
http://dx.doi.org/10.1186/s13578-021-00618-0