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Choice of 16S ribosomal RNA primers affects the microbiome analysis in chicken ceca

We evaluated the effect of applying different sets of 16S rRNA primers on bacterial composition, diversity, and predicted function in chicken ceca. Cecal contents from Ross 708 birds at 1, 3, and 5 weeks of age were collected for DNA isolation. Eight different primer pairs targeting different variab...

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Detalles Bibliográficos
Autores principales: Darwish, Nadia, Shao, Jonathan, Schreier, Lori L., Proszkowiec-Weglarz, Monika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178357/
https://www.ncbi.nlm.nih.gov/pubmed/34088939
http://dx.doi.org/10.1038/s41598-021-91387-w