Enhancement of protein thermostability by three consecutive mutations using loop-walking method and machine learning

We developed a method to improve protein thermostability, “loop-walking method”. Three consecutive positions in 12 loops of Burkholderia cepacia lipase were subjected to random mutagenesis to make 12 libraries. Screening allowed us to identify L7 as a hot-spot loop having an impact on thermostabilit...

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Detalles Bibliográficos
Autores principales: Yoshida, Kazunori, Kawai, Shun, Fujitani, Masaya, Koikeda, Satoshi, Kato, Ryuji, Ema, Tadashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178419/
https://www.ncbi.nlm.nih.gov/pubmed/34088952
http://dx.doi.org/10.1038/s41598-021-91339-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178419/
https://www.ncbi.nlm.nih.gov/pubmed/34088952
http://dx.doi.org/10.1038/s41598-021-91339-4

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