Ultrasensitive single-cell proteomics workflow identifies >1000 protein groups per mammalian cell

We report on the combination of nanodroplet sample preparation, ultra-low-flow nanoLC, high-field asymmetric ion mobility spectrometry (FAIMS), and the latest-generation Orbitrap Eclipse Tribrid mass spectrometer for greatly improved single-cell proteome profiling. FAIMS effectively filtered out sin...

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Detalles Bibliográficos
Autores principales: Cong, Yongzheng, Motamedchaboki, Khatereh, Misal, Santosh A., Liang, Yiran, Guise, Amanda J., Truong, Thy, Huguet, Romain, Plowey, Edward D., Zhu, Ying, Lopez-Ferrer, Daniel, Kelly, Ryan T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178986/
https://www.ncbi.nlm.nih.gov/pubmed/34163866
http://dx.doi.org/10.1039/d0sc03636f

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178986/
https://www.ncbi.nlm.nih.gov/pubmed/34163866
http://dx.doi.org/10.1039/d0sc03636f

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