Cargando…

A novel catalytic heme cofactor in SfmD with a single thioether bond and a bis-His ligand set revealed by a de novo crystal structural and spectroscopic study

SfmD is a heme-dependent enzyme in the biosynthetic pathway of saframycin A. Here, we present a 1.78 Å resolution de novo crystal structure of SfmD, which unveils a novel heme cofactor attached to the protein with an unusual Hx(n)HxxxC motif (n ∼ 38). This heme cofactor is unique in two respects. It...

Descripción completa

Detalles Bibliográficos
Autores principales: Shin, Inchul, Davis, Ian, Nieves-Merced, Karinel, Wang, Yifan, McHardy, Stanton, Liu, Aimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179489/
https://www.ncbi.nlm.nih.gov/pubmed/34163669
http://dx.doi.org/10.1039/d0sc06369j