One‐step selective affinity purification and immobilization of His‐tagged enzyme by recyclable magnetic nanoparticles

The NiFe(2)O(4) magnetic nanoparticles (NF‐MNPs) were prepared for one‐step selective affinity purification and immobilization of His‐tagged recombinant glucose dehydrogenase (GluDH). The prepared nanoparticles were characterized by a Fourier‐transform infrared spectrophotometer and microscopy. The...

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Detalles Bibliográficos
Autores principales: Zhou, Li‐Jian, Li, Rui‐Fang, Li, Xue‐Yong, Zhang, Ye‐Wang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182278/
https://www.ncbi.nlm.nih.gov/pubmed/34140847
http://dx.doi.org/10.1002/elsc.202000093

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182278/
https://www.ncbi.nlm.nih.gov/pubmed/34140847
http://dx.doi.org/10.1002/elsc.202000093

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