Cargando…

One‐step selective affinity purification and immobilization of His‐tagged enzyme by recyclable magnetic nanoparticles

The NiFe(2)O(4) magnetic nanoparticles (NF‐MNPs) were prepared for one‐step selective affinity purification and immobilization of His‐tagged recombinant glucose dehydrogenase (GluDH). The prepared nanoparticles were characterized by a Fourier‐transform infrared spectrophotometer and microscopy. The...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zhou, Li‐Jian, Li, Rui‐Fang, Li, Xue‐Yong, Zhang, Ye‐Wang
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2021
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182278/ https://www.ncbi.nlm.nih.gov/pubmed/34140847 http://dx.doi.org/10.1002/elsc.202000093

Ejemplares similares

Magnetic One-Step Purification of His-Tagged Protein by Bare Iron Oxide Nanoparticles
por: Schwaminger, Sebastian P., et al.
Publicado: (2019)

Engineering His-Tagged Senecavirus A for One-Step Purification of Viral Antigens
por: Fan, Junhao, et al.
Publicado: (2022)

Selective Immobilization of His-Tagged Enzyme on Ni-Chelated Ion Exchange Resin and Its Application in Protein Purification
por: Wang, Kangjing, et al.
Publicado: (2023)

Synchronized purification and immobilization of his-tagged β-glucosidase via Fe(3)O(4)/PMG core/shell magnetic nanoparticles
por: Zhou, Yang, et al.
Publicado: (2017)

Ni-modified magnetic nanoparticles for affinity purification of His-tagged proteins from the complex matrix of the silkworm fat body
por: Minkner, Robert, et al.
Publicado: (2020)

One-step purification and immobilization of extracellularly expressed sortase A by magnetic particles to develop a robust and recyclable biocatalyst
por: Zhao, Xinrui, et al.
Publicado: (2017)

Immobilization and Purification of Enzymes With the Novel Affinity Tag ChBD-AB From Chitinolyticbacter meiyuanensis SYBC-H1
por: Zhou, Jie, et al.
Publicado: (2020)

Cloning, expression, and one-step purification/immobilization of two carbohydrate-binding module-tagged alcohol dehydrogenases
por: Benito, Mario, et al.
Publicado: (2022)

Novel Application of Magnetic Protein: Convenient One-Step Purification and Immobilization of Proteins
por: Jiang, Min, et al.
Publicado: (2017)

Co-Immobilization of Enzymes and Magnetic Nanoparticles by Metal-Nucleotide Hydrogelnanofibers for Improving Stability and Recycling
por: Li, Chunfang, et al.
Publicado: (2017)

An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography
por: Scheich, Christoph, et al.
Publicado: (2003)

Characterization of Recombinant His-Tag Protein Immobilized onto Functionalized Gold Nanoparticles
por: Torres-González, Lisa, et al.
Publicado: (2018)

Synthesis of magnetic nanoparticles with an IDA or TED modified surface for purification and immobilization of poly-histidine tagged proteins
por: Zeng, Kai, et al.
Publicado: (2020)

Enzyme Storage and Recycling: Nanoassemblies of α-Amylase and Xylanase Immobilized on Biomimetic Magnetic Nanoparticles
por: Salem, Karima, et al.
Publicado: (2021)

Several Affinity Tags Commonly Used in Chromatographic Purification
por: Zhao, Xinyu, et al.
Publicado: (2013)

Peptide-Mediated Immobilization on Magnetoferritin for Enzyme Recycling
por: Zhang, Yu, et al.
Publicado: (2019)

Tag-mediated single-step purification and immobilization of recombinant proteins toward protein-engineered advanced materials
por: Freitas, Ana I., et al.
Publicado: (2021)

Affinity Purification of Angiotensin Converting Enzyme Inhibitory Peptides from Wakame (Undaria Pinnatifida) Using Immobilized ACE on Magnetic Metal Organic Frameworks
por: Feng, Xuezhen, et al.
Publicado: (2021)

Development of alkaline phosphatase-scFv and its use for one-step enzyme-linked immunosorbent assay for His-tagged protein detection
por: He, Shuzhen, et al.
Publicado: (2022)

High-throughput Purification of Affinity-tagged Recombinant Proteins
por: Wiesler, Simone C., et al.
Publicado: (2012)

Preparative SDS PAGE as an Alternative to His-Tag Purification of Recombinant Amelogenin
por: Gabe, Claire M., et al.
Publicado: (2017)

A single-step method for purification of active His-tagged ribosomes from a genetically engineered Escherichia coli
por: Ederth, Josefine, et al.
Publicado: (2009)

Recombinant production and affinity purification of the FraC pore forming toxin using hexa-His tag and pET expression cassette
por: Imani, Mehdi, et al.
Publicado: (2017)

Alternative application of an affinity purification tag: hexahistidines in ester hydrolysis
por: Schoonen, Lise, et al.
Publicado: (2017)

Oriented Immobilization and Quantitative Analysis Simultaneously Realized in Sandwich Immunoassay via His-Tagged Nanobody
por: Xu, Li, et al.
Publicado: (2019)

High Affinity Immobilization of Proteins Using the CrAsH/TC Tag
por: Schulte-Zweckel, Janine, et al.
Publicado: (2016)

Expression and Purification of Recombinant Proteins in Escherichia coli with a His(6) or Dual His(6)-MBP Tag
por: Raran-Kurussi, Sreejith, et al.
Publicado: (2017)

Methylamine-Sensitive Amperometric Biosensor Based on (His)(6)-Tagged Hansenula polymorpha Methylamine Oxidase Immobilized on the Gold Nanoparticles
por: Stasyuk, Nataliya Ye., et al.
Publicado: (2014)

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
por: Nilvebrant, Johan, et al.
Publicado: (2012)

Purification of Affinity Tag-free Recombinant Tubulin from Insect Cells
por: Ti, Shih-Chieh, et al.
Publicado: (2020)

The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides
por: Bartnicki, Filip, et al.
Publicado: (2017)

Large-scale purification and crystallization of the endoribonuclease XendoU: troubleshooting with His-tagged proteins
por: Renzi, Fabiana, et al.
Publicado: (2006)

Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
por: Kim, Ji Hun, et al.
Publicado: (2010)

One-plasmid double-expression His-tag system for rapid production and easy purification of MS2 phage-like particles
por: Mikel, Pavel, et al.
Publicado: (2017)

Starch-Coated Magnetic Iron Oxide Nanoparticles for Affinity Purification of Recombinant Proteins
por: Krasitskaya, Vasilisa V., et al.
Publicado: (2022)

High level expression of nicotinamide nucleoside kinase from Saccharomyces cerevisiae and its purification and immobilization by one-step method
por: He, Jian-Ju, et al.
Publicado: (2023)

Antibody-Free Magnetic Cell Sorting of Genetically Modified Primary Human CD4+ T Cells by One-Step Streptavidin Affinity Purification
por: Matheson, Nicholas J., et al.
Publicado: (2014)

Facile and high-efficient immobilization of histidine-tagged multimeric protein G on magnetic nanoparticles
por: Lee, Jiho, et al.
Publicado: (2014)

Substrate Specificity and Enzyme Recycling Using Chitosan Immobilized Laccase
por: Skoronski, Everton, et al.
Publicado: (2014)

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
por: Luzarowski, Marcin, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_7emnq3r4j57e0l6ho2s55rpl9e