Blocking Cross-Species Secondary Binding When Performing Double Immunostaining With Mouse and Rat Primary Antibodies

Immunostaining is a powerful technique and widely used to identify molecules in tissues and cells, although critical steps are necessary to block cross-reaction. Here we focused on an overlooked cross immunoreactivity issue where a secondary antibody (secondary) cross-reacts with a primary antibody...

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Detalles Bibliográficos
Autores principales: Mao, Shanping, Xiong, Guoxiang, Johnson, Brian N., Cohen, Noam A., Cohen, Akiva S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185172/
https://www.ncbi.nlm.nih.gov/pubmed/34113227
http://dx.doi.org/10.3389/fnins.2021.579859

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185172/
https://www.ncbi.nlm.nih.gov/pubmed/34113227
http://dx.doi.org/10.3389/fnins.2021.579859

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