Combined single-molecule fluorescence in situ hybridization and immunohistochemistry analysis in intact murine dorsal root ganglia and sciatic nerve

Single-molecule fluorescence in situ hybridization (smFISH) allows spatial mapping of gene expression. This protocol presents advances in smFISH fidelity and flexibility in intact murine sensory nervous system tissue. An approach using RNAscope probes allows multiplexing, enhanced target specificity...

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Detalles Bibliográficos
Autores principales: Li, Xinbei, Eadara, Sreenivas, Jeon, Sangmin, Liu, Yan, Muwanga, Gabriella, Qu, Lintao, Caterina, Michael J., Meffert, Mollie K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185307/
https://www.ncbi.nlm.nih.gov/pubmed/34142098
http://dx.doi.org/10.1016/j.xpro.2021.100555

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185307/
https://www.ncbi.nlm.nih.gov/pubmed/34142098
http://dx.doi.org/10.1016/j.xpro.2021.100555

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