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Ultrafast enzymatic digestion of deoxyribonucleic acid in aqueous microdroplets for sequence discrimination and identification

We report the use of aqueous microdroplets to accelerate deoxyribonucleic acid (DNA) fragmentation by deoxyribonuclease I (DNase I), and we present a simple, ultrafast approach named DNA fragment mass fingerprinting to discriminate different DNA sequences by comparing their fragment mass patterns. D...

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Detalles Bibliográficos
Autores principales: Zhong, Xiaoqin, Chen, Hao, Zare, Richard N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cambridge University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185430/
https://www.ncbi.nlm.nih.gov/pubmed/34192264
http://dx.doi.org/10.1017/qrd.2021.2