A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses

The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We demonstrate that despite the large size of the viral RNA genome (~30 kb), infectious full-length cDNA is readily assembled in vitro by a circular polymerase extension reaction (CPER) metho...

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Detalles Bibliográficos
Autores principales: Amarilla, Alberto A., Sng, Julian D. J., Parry, Rhys, Deerain, Joshua M., Potter, James R., Setoh, Yin Xiang, Rawle, Daniel J., Le, Thuy T., Modhiran, Naphak, Wang, Xiaohui, Peng, Nias Y. G., Torres, Francisco J., Pyke, Alyssa, Harrison, Jessica J., Freney, Morgan E., Liang, Benjamin, McMillan, Christopher L. D., Cheung, Stacey T. M., Guevara, Darwin J. Da Costa, Hardy, Joshua M., Bettington, Mark, Muller, David A., Coulibaly, Fasséli, Moore, Frederick, Hall, Roy A., Young, Paul R., Mackenzie, Jason M., Hobson-Peters, Jody, Suhrbier, Andreas, Watterson, Daniel, Khromykh, Alexander A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187723/
https://www.ncbi.nlm.nih.gov/pubmed/34103499
http://dx.doi.org/10.1038/s41467-021-23779-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187723/
https://www.ncbi.nlm.nih.gov/pubmed/34103499
http://dx.doi.org/10.1038/s41467-021-23779-5

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