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Stalling chromophore synthesis of the fluorescent protein Venus reveals the molecular basis of the final oxidation step
Fluorescent proteins (FPs) have revolutionised the life sciences, but the mechanism of chromophore maturation is still not fully understood. Here we show that incorporation of a photo-responsive non-canonical amino acid within the chromophore stalls maturation of Venus, a yellow FP, at an intermedia...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The Royal Society of Chemistry
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188506/ https://www.ncbi.nlm.nih.gov/pubmed/34168826 http://dx.doi.org/10.1039/d0sc06693a |