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Generation of locus-specific degradable tag knock-ins in mouse and human cell lines

Protein degradation technologies represent a powerful functional genomics tool, allowing fast and controllable target protein depletion. Establishing these systems requires a knock-in of the degradation tag into both endogenous target gene alleles. Here, we provide a step-by-step protocol for the ef...

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Detalles Bibliográficos
Autores principales: Damhofer, Helene, Radzisheuskaya, Aliaksandra, Helin, Kristian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188616/
https://www.ncbi.nlm.nih.gov/pubmed/34151298
http://dx.doi.org/10.1016/j.xpro.2021.100575