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Generation of locus-specific degradable tag knock-ins in mouse and human cell lines
Protein degradation technologies represent a powerful functional genomics tool, allowing fast and controllable target protein depletion. Establishing these systems requires a knock-in of the degradation tag into both endogenous target gene alleles. Here, we provide a step-by-step protocol for the ef...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188616/ https://www.ncbi.nlm.nih.gov/pubmed/34151298 http://dx.doi.org/10.1016/j.xpro.2021.100575 |