Differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions

We describe the extended endothelial cell culture method (EECM) for the differentiation of human pluripotent stem cells (hPSCs) into brain microvascular endothelial cell (BMEC)-like cells. EECM-BMEC-like cells resemble primary human BMECs in morphology, molecular junctional architecture, and diffusi...

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Detalles Bibliográficos
Autores principales: Nishihara, Hideaki, Gastfriend, Benjamin D., Kasap, Pelin, Palecek, Sean P., Shusta, Eric V., Engelhardt, Britta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188617/
https://www.ncbi.nlm.nih.gov/pubmed/34151293
http://dx.doi.org/10.1016/j.xpro.2021.100563
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author Nishihara, Hideaki
Gastfriend, Benjamin D.
Kasap, Pelin
Palecek, Sean P.
Shusta, Eric V.
Engelhardt, Britta
author_facet Nishihara, Hideaki
Gastfriend, Benjamin D.
Kasap, Pelin
Palecek, Sean P.
Shusta, Eric V.
Engelhardt, Britta
author_sort Nishihara, Hideaki
collection PubMed
description We describe the extended endothelial cell culture method (EECM) for the differentiation of human pluripotent stem cells (hPSCs) into brain microvascular endothelial cell (BMEC)-like cells. EECM-BMEC-like cells resemble primary human BMECs in morphology, molecular junctional architecture, and diffusion barrier characteristics. A mature immune phenotype with proper endothelial adhesion molecule expression makes this model distinct from any other hPSC-derived in vitro blood-brain barrier (BBB) model and suitable to study immune cell migration across the BBB in a disease relevant and personalized fashion. For complete details on the use and execution of this protocol, please refer to Lian et al. (2014), Nishihara et al. (2020a).
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spelling pubmed-81886172021-06-17 Differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions Nishihara, Hideaki Gastfriend, Benjamin D. Kasap, Pelin Palecek, Sean P. Shusta, Eric V. Engelhardt, Britta STAR Protoc Protocol We describe the extended endothelial cell culture method (EECM) for the differentiation of human pluripotent stem cells (hPSCs) into brain microvascular endothelial cell (BMEC)-like cells. EECM-BMEC-like cells resemble primary human BMECs in morphology, molecular junctional architecture, and diffusion barrier characteristics. A mature immune phenotype with proper endothelial adhesion molecule expression makes this model distinct from any other hPSC-derived in vitro blood-brain barrier (BBB) model and suitable to study immune cell migration across the BBB in a disease relevant and personalized fashion. For complete details on the use and execution of this protocol, please refer to Lian et al. (2014), Nishihara et al. (2020a). Elsevier 2021-06-02 /pmc/articles/PMC8188617/ /pubmed/34151293 http://dx.doi.org/10.1016/j.xpro.2021.100563 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Nishihara, Hideaki
Gastfriend, Benjamin D.
Kasap, Pelin
Palecek, Sean P.
Shusta, Eric V.
Engelhardt, Britta
Differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions
title Differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions
title_full Differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions
title_fullStr Differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions
title_full_unstemmed Differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions
title_short Differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions
title_sort differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188617/
https://www.ncbi.nlm.nih.gov/pubmed/34151293
http://dx.doi.org/10.1016/j.xpro.2021.100563
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