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Protocols for analysis of mitochondrial permeability transition pore opening in mouse somatic cell reprogramming
Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. Here, we describe a protocol for imaging mitochondrial permeability transition pore (mPTP) opening in reprogramming of somatic cells using a confocal microscope. We also describe a method to sort high a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188620/ https://www.ncbi.nlm.nih.gov/pubmed/34151295 http://dx.doi.org/10.1016/j.xpro.2021.100568 |