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Protocols for analysis of mitochondrial permeability transition pore opening in mouse somatic cell reprogramming

Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. Here, we describe a protocol for imaging mitochondrial permeability transition pore (mPTP) opening in reprogramming of somatic cells using a confocal microscope. We also describe a method to sort high a...

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Detalles Bibliográficos
Autores principales: Ying, Zhongfu, Liu, Zihuang, Xiang, Ge, Xin, Yanmin, Wang, Junwei, Liu, Xingguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188620/
https://www.ncbi.nlm.nih.gov/pubmed/34151295
http://dx.doi.org/10.1016/j.xpro.2021.100568