A chromosome-level Camptotheca acuminata genome assembly provides insights into the evolutionary origin of camptothecin biosynthesis

Camptothecin and its derivatives are widely used for treating malignant tumors. Previous studies revealed only a limited number of candidate genes for camptothecin biosynthesis in Camptotheca acuminata, and it is still poorly understood how its biosynthesis of camptothecin has evolved. Here, we repo...

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Autores principales: Kang, Minghui, Fu, Rao, Zhang, Pingyu, Lou, Shangling, Yang, Xuchen, Chen, Yang, Ma, Tao, Zhang, Yang, Xi, Zhenxiang, Liu, Jianquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192753/
https://www.ncbi.nlm.nih.gov/pubmed/34112794
http://dx.doi.org/10.1038/s41467-021-23872-9
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author Kang, Minghui
Fu, Rao
Zhang, Pingyu
Lou, Shangling
Yang, Xuchen
Chen, Yang
Ma, Tao
Zhang, Yang
Xi, Zhenxiang
Liu, Jianquan
author_facet Kang, Minghui
Fu, Rao
Zhang, Pingyu
Lou, Shangling
Yang, Xuchen
Chen, Yang
Ma, Tao
Zhang, Yang
Xi, Zhenxiang
Liu, Jianquan
author_sort Kang, Minghui
collection PubMed
description Camptothecin and its derivatives are widely used for treating malignant tumors. Previous studies revealed only a limited number of candidate genes for camptothecin biosynthesis in Camptotheca acuminata, and it is still poorly understood how its biosynthesis of camptothecin has evolved. Here, we report a high-quality, chromosome-level C. acuminata genome assembly. We find that C. acuminata experiences an independent whole-genome duplication and numerous genes derive from it are related to camptothecin biosynthesis. Comparing with Catharanthus roseus, the loganic acid O-methyltransferase (LAMT) in C. acuminata fails to convert loganic acid into loganin. Instead, two secologanic acid synthases (SLASs) convert loganic acid to secologanic acid. The functional divergence of the LAMT gene and positive evolution of two SLAS genes, therefore, both contribute greatly to the camptothecin biosynthesis in C. acuminata. Our results emphasize the importance of high-quality genome assembly in identifying genetic changes in the evolutionary origin of a secondary metabolite.
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spelling pubmed-81927532021-06-17 A chromosome-level Camptotheca acuminata genome assembly provides insights into the evolutionary origin of camptothecin biosynthesis Kang, Minghui Fu, Rao Zhang, Pingyu Lou, Shangling Yang, Xuchen Chen, Yang Ma, Tao Zhang, Yang Xi, Zhenxiang Liu, Jianquan Nat Commun Article Camptothecin and its derivatives are widely used for treating malignant tumors. Previous studies revealed only a limited number of candidate genes for camptothecin biosynthesis in Camptotheca acuminata, and it is still poorly understood how its biosynthesis of camptothecin has evolved. Here, we report a high-quality, chromosome-level C. acuminata genome assembly. We find that C. acuminata experiences an independent whole-genome duplication and numerous genes derive from it are related to camptothecin biosynthesis. Comparing with Catharanthus roseus, the loganic acid O-methyltransferase (LAMT) in C. acuminata fails to convert loganic acid into loganin. Instead, two secologanic acid synthases (SLASs) convert loganic acid to secologanic acid. The functional divergence of the LAMT gene and positive evolution of two SLAS genes, therefore, both contribute greatly to the camptothecin biosynthesis in C. acuminata. Our results emphasize the importance of high-quality genome assembly in identifying genetic changes in the evolutionary origin of a secondary metabolite. Nature Publishing Group UK 2021-06-10 /pmc/articles/PMC8192753/ /pubmed/34112794 http://dx.doi.org/10.1038/s41467-021-23872-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Kang, Minghui
Fu, Rao
Zhang, Pingyu
Lou, Shangling
Yang, Xuchen
Chen, Yang
Ma, Tao
Zhang, Yang
Xi, Zhenxiang
Liu, Jianquan
A chromosome-level Camptotheca acuminata genome assembly provides insights into the evolutionary origin of camptothecin biosynthesis
title A chromosome-level Camptotheca acuminata genome assembly provides insights into the evolutionary origin of camptothecin biosynthesis
title_full A chromosome-level Camptotheca acuminata genome assembly provides insights into the evolutionary origin of camptothecin biosynthesis
title_fullStr A chromosome-level Camptotheca acuminata genome assembly provides insights into the evolutionary origin of camptothecin biosynthesis
title_full_unstemmed A chromosome-level Camptotheca acuminata genome assembly provides insights into the evolutionary origin of camptothecin biosynthesis
title_short A chromosome-level Camptotheca acuminata genome assembly provides insights into the evolutionary origin of camptothecin biosynthesis
title_sort chromosome-level camptotheca acuminata genome assembly provides insights into the evolutionary origin of camptothecin biosynthesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192753/
https://www.ncbi.nlm.nih.gov/pubmed/34112794
http://dx.doi.org/10.1038/s41467-021-23872-9
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