Type I and III IFNs produced by the nasal epithelia and dimmed inflammation are features of alpacas resolving MERS-CoV infection
While MERS-CoV (Middle East respiratory syndrome Coronavirus) provokes a lethal disease in humans, camelids, the main virus reservoir, are asymptomatic carriers, suggesting a crucial role for innate immune responses in controlling the infection. Experimentally infected camelids clear infectious viru...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195365/ https://www.ncbi.nlm.nih.gov/pubmed/34029358 http://dx.doi.org/10.1371/journal.ppat.1009229 |
_version_ | 1783706486438887424 |
---|---|
author | Te, Nigeer Rodon, Jordi Ballester, Maria Pérez, Mónica Pailler-García, Lola Segalés, Joaquim Vergara-Alert, Júlia Bensaid, Albert |
author_facet | Te, Nigeer Rodon, Jordi Ballester, Maria Pérez, Mónica Pailler-García, Lola Segalés, Joaquim Vergara-Alert, Júlia Bensaid, Albert |
author_sort | Te, Nigeer |
collection | PubMed |
description | While MERS-CoV (Middle East respiratory syndrome Coronavirus) provokes a lethal disease in humans, camelids, the main virus reservoir, are asymptomatic carriers, suggesting a crucial role for innate immune responses in controlling the infection. Experimentally infected camelids clear infectious virus within one week and mount an effective adaptive immune response. Here, transcription of immune response genes was monitored in the respiratory tract of MERS-CoV infected alpacas. Concomitant to the peak of infection, occurring at 2 days post inoculation (dpi), type I and III interferons (IFNs) were maximally transcribed only in the nasal mucosa of alpacas, while interferon stimulated genes (ISGs) were induced along the whole respiratory tract. Simultaneous to mild focal infiltration of leukocytes in nasal mucosa and submucosa, upregulation of the anti-inflammatory cytokine IL10 and dampened transcription of pro-inflammatory genes under NF-κB control were observed. In the lung, early (1 dpi) transcription of chemokines (CCL2 and CCL3) correlated with a transient accumulation of mainly mononuclear leukocytes. A tight regulation of IFNs in lungs with expression of ISGs and controlled inflammatory responses, might contribute to virus clearance without causing tissue damage. Thus, the nasal mucosa, the main target of MERS-CoV in camelids, seems central in driving an efficient innate immune response based on triggering ISGs as well as the dual anti-inflammatory effects of type III IFNs and IL10. |
format | Online Article Text |
id | pubmed-8195365 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-81953652021-06-21 Type I and III IFNs produced by the nasal epithelia and dimmed inflammation are features of alpacas resolving MERS-CoV infection Te, Nigeer Rodon, Jordi Ballester, Maria Pérez, Mónica Pailler-García, Lola Segalés, Joaquim Vergara-Alert, Júlia Bensaid, Albert PLoS Pathog Research Article While MERS-CoV (Middle East respiratory syndrome Coronavirus) provokes a lethal disease in humans, camelids, the main virus reservoir, are asymptomatic carriers, suggesting a crucial role for innate immune responses in controlling the infection. Experimentally infected camelids clear infectious virus within one week and mount an effective adaptive immune response. Here, transcription of immune response genes was monitored in the respiratory tract of MERS-CoV infected alpacas. Concomitant to the peak of infection, occurring at 2 days post inoculation (dpi), type I and III interferons (IFNs) were maximally transcribed only in the nasal mucosa of alpacas, while interferon stimulated genes (ISGs) were induced along the whole respiratory tract. Simultaneous to mild focal infiltration of leukocytes in nasal mucosa and submucosa, upregulation of the anti-inflammatory cytokine IL10 and dampened transcription of pro-inflammatory genes under NF-κB control were observed. In the lung, early (1 dpi) transcription of chemokines (CCL2 and CCL3) correlated with a transient accumulation of mainly mononuclear leukocytes. A tight regulation of IFNs in lungs with expression of ISGs and controlled inflammatory responses, might contribute to virus clearance without causing tissue damage. Thus, the nasal mucosa, the main target of MERS-CoV in camelids, seems central in driving an efficient innate immune response based on triggering ISGs as well as the dual anti-inflammatory effects of type III IFNs and IL10. Public Library of Science 2021-05-24 /pmc/articles/PMC8195365/ /pubmed/34029358 http://dx.doi.org/10.1371/journal.ppat.1009229 Text en © 2021 Te et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Te, Nigeer Rodon, Jordi Ballester, Maria Pérez, Mónica Pailler-García, Lola Segalés, Joaquim Vergara-Alert, Júlia Bensaid, Albert Type I and III IFNs produced by the nasal epithelia and dimmed inflammation are features of alpacas resolving MERS-CoV infection |
title | Type I and III IFNs produced by the nasal epithelia and dimmed inflammation are features of alpacas resolving MERS-CoV infection |
title_full | Type I and III IFNs produced by the nasal epithelia and dimmed inflammation are features of alpacas resolving MERS-CoV infection |
title_fullStr | Type I and III IFNs produced by the nasal epithelia and dimmed inflammation are features of alpacas resolving MERS-CoV infection |
title_full_unstemmed | Type I and III IFNs produced by the nasal epithelia and dimmed inflammation are features of alpacas resolving MERS-CoV infection |
title_short | Type I and III IFNs produced by the nasal epithelia and dimmed inflammation are features of alpacas resolving MERS-CoV infection |
title_sort | type i and iii ifns produced by the nasal epithelia and dimmed inflammation are features of alpacas resolving mers-cov infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195365/ https://www.ncbi.nlm.nih.gov/pubmed/34029358 http://dx.doi.org/10.1371/journal.ppat.1009229 |
work_keys_str_mv | AT tenigeer typeiandiiiifnsproducedbythenasalepitheliaanddimmedinflammationarefeaturesofalpacasresolvingmerscovinfection AT rodonjordi typeiandiiiifnsproducedbythenasalepitheliaanddimmedinflammationarefeaturesofalpacasresolvingmerscovinfection AT ballestermaria typeiandiiiifnsproducedbythenasalepitheliaanddimmedinflammationarefeaturesofalpacasresolvingmerscovinfection AT perezmonica typeiandiiiifnsproducedbythenasalepitheliaanddimmedinflammationarefeaturesofalpacasresolvingmerscovinfection AT paillergarcialola typeiandiiiifnsproducedbythenasalepitheliaanddimmedinflammationarefeaturesofalpacasresolvingmerscovinfection AT segalesjoaquim typeiandiiiifnsproducedbythenasalepitheliaanddimmedinflammationarefeaturesofalpacasresolvingmerscovinfection AT vergaraalertjulia typeiandiiiifnsproducedbythenasalepitheliaanddimmedinflammationarefeaturesofalpacasresolvingmerscovinfection AT bensaidalbert typeiandiiiifnsproducedbythenasalepitheliaanddimmedinflammationarefeaturesofalpacasresolvingmerscovinfection |