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PET/CT targeted tissue sampling reveals virus specific dIgA can alter the distribution and localization of HIV after rectal exposure
Human immunodeficiency virus (HIV) vaccines have not been successful in clinical trials. Dimeric IgA (dIgA) in the form of secretory IgA is the most abundant antibody class in mucosal tissues, making dIgA a prime candidate for potential HIV vaccines. We coupled Positron Emission Tomography (PET) ima...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195437/ https://www.ncbi.nlm.nih.gov/pubmed/34061907 http://dx.doi.org/10.1371/journal.ppat.1009632 |
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author | Taylor, Roslyn A. Xiao, Sixia Carias, Ann M. McRaven, Michael D. Thakkar, Divya N. Araínga, Mariluz Allen, Edward J. Rogers, Kenneth A. Kumarapperuma, Sidath C. Gong, Siqi Fought, Angela J. Anderson, Meegan R. Thomas, Yanique Schneider, Jeffrey R. Goins, Beth Fox, Peter Villinger, Francois J. Ruprecht, Ruth M. Hope, Thomas J. |
author_facet | Taylor, Roslyn A. Xiao, Sixia Carias, Ann M. McRaven, Michael D. Thakkar, Divya N. Araínga, Mariluz Allen, Edward J. Rogers, Kenneth A. Kumarapperuma, Sidath C. Gong, Siqi Fought, Angela J. Anderson, Meegan R. Thomas, Yanique Schneider, Jeffrey R. Goins, Beth Fox, Peter Villinger, Francois J. Ruprecht, Ruth M. Hope, Thomas J. |
author_sort | Taylor, Roslyn A. |
collection | PubMed |
description | Human immunodeficiency virus (HIV) vaccines have not been successful in clinical trials. Dimeric IgA (dIgA) in the form of secretory IgA is the most abundant antibody class in mucosal tissues, making dIgA a prime candidate for potential HIV vaccines. We coupled Positron Emission Tomography (PET) imaging and fluorescent microscopy of (64)Cu-labeled, photoactivatable-GFP HIV (PA-GFP-BaL) and fluorescently labeled dIgA to determine how dIgA antibodies influence virus interaction with mucosal barriers and viral penetration in colorectal tissue. Our results show that HIV virions rapidly disseminate throughout the colon two hours after exposure. The presence of dIgA resulted in an increase in virions and penetration depth in the transverse colon. Moreover, virions were found in the mesenteric lymph nodes two hours after viral exposure, and the presence of dIgA led to an increase in virions in mesenteric lymph nodes. Taken together, these technologies enable in vivo and in situ visualization of antibody-virus interactions and detailed investigations of early events in HIV infection. |
format | Online Article Text |
id | pubmed-8195437 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-81954372021-06-21 PET/CT targeted tissue sampling reveals virus specific dIgA can alter the distribution and localization of HIV after rectal exposure Taylor, Roslyn A. Xiao, Sixia Carias, Ann M. McRaven, Michael D. Thakkar, Divya N. Araínga, Mariluz Allen, Edward J. Rogers, Kenneth A. Kumarapperuma, Sidath C. Gong, Siqi Fought, Angela J. Anderson, Meegan R. Thomas, Yanique Schneider, Jeffrey R. Goins, Beth Fox, Peter Villinger, Francois J. Ruprecht, Ruth M. Hope, Thomas J. PLoS Pathog Research Article Human immunodeficiency virus (HIV) vaccines have not been successful in clinical trials. Dimeric IgA (dIgA) in the form of secretory IgA is the most abundant antibody class in mucosal tissues, making dIgA a prime candidate for potential HIV vaccines. We coupled Positron Emission Tomography (PET) imaging and fluorescent microscopy of (64)Cu-labeled, photoactivatable-GFP HIV (PA-GFP-BaL) and fluorescently labeled dIgA to determine how dIgA antibodies influence virus interaction with mucosal barriers and viral penetration in colorectal tissue. Our results show that HIV virions rapidly disseminate throughout the colon two hours after exposure. The presence of dIgA resulted in an increase in virions and penetration depth in the transverse colon. Moreover, virions were found in the mesenteric lymph nodes two hours after viral exposure, and the presence of dIgA led to an increase in virions in mesenteric lymph nodes. Taken together, these technologies enable in vivo and in situ visualization of antibody-virus interactions and detailed investigations of early events in HIV infection. Public Library of Science 2021-06-01 /pmc/articles/PMC8195437/ /pubmed/34061907 http://dx.doi.org/10.1371/journal.ppat.1009632 Text en © 2021 Taylor et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Taylor, Roslyn A. Xiao, Sixia Carias, Ann M. McRaven, Michael D. Thakkar, Divya N. Araínga, Mariluz Allen, Edward J. Rogers, Kenneth A. Kumarapperuma, Sidath C. Gong, Siqi Fought, Angela J. Anderson, Meegan R. Thomas, Yanique Schneider, Jeffrey R. Goins, Beth Fox, Peter Villinger, Francois J. Ruprecht, Ruth M. Hope, Thomas J. PET/CT targeted tissue sampling reveals virus specific dIgA can alter the distribution and localization of HIV after rectal exposure |
title | PET/CT targeted tissue sampling reveals virus specific dIgA can alter the distribution and localization of HIV after rectal exposure |
title_full | PET/CT targeted tissue sampling reveals virus specific dIgA can alter the distribution and localization of HIV after rectal exposure |
title_fullStr | PET/CT targeted tissue sampling reveals virus specific dIgA can alter the distribution and localization of HIV after rectal exposure |
title_full_unstemmed | PET/CT targeted tissue sampling reveals virus specific dIgA can alter the distribution and localization of HIV after rectal exposure |
title_short | PET/CT targeted tissue sampling reveals virus specific dIgA can alter the distribution and localization of HIV after rectal exposure |
title_sort | pet/ct targeted tissue sampling reveals virus specific diga can alter the distribution and localization of hiv after rectal exposure |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195437/ https://www.ncbi.nlm.nih.gov/pubmed/34061907 http://dx.doi.org/10.1371/journal.ppat.1009632 |
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