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Allele frequencies of single nucleotide polymorphisms of clinically important drug-metabolizing enzymes CYP2C9, CYP2C19, and CYP3A4 in a Thai population

Prior knowledge of allele frequencies of cytochrome P450 polymorphisms in a population is crucial for the revision and optimization of existing medication choices and doses. In the current study, the frequency of the CYP2C9*2, CYP2C9*3, CYP2C19*2, CYP2C19*3, CYP2C19*6, CYP2C19*17, and CYP3A4 (rs4646...

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Autores principales: Sukprasong, Rattanaporn, Chuwongwattana, Sumonrat, Koomdee, Napatrupron, Jantararoungtong, Thawinee, Prommas, Santirhat, Jinda, Pimonpan, Rachanakul, Jiratha, Nuntharadthanaphong, Nutthan, Jongjitsook, Nutcha, Puangpetch, Apichaya, Sukasem, Chonlaphat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195986/
https://www.ncbi.nlm.nih.gov/pubmed/34117307
http://dx.doi.org/10.1038/s41598-021-90969-y
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author Sukprasong, Rattanaporn
Chuwongwattana, Sumonrat
Koomdee, Napatrupron
Jantararoungtong, Thawinee
Prommas, Santirhat
Jinda, Pimonpan
Rachanakul, Jiratha
Nuntharadthanaphong, Nutthan
Jongjitsook, Nutcha
Puangpetch, Apichaya
Sukasem, Chonlaphat
author_facet Sukprasong, Rattanaporn
Chuwongwattana, Sumonrat
Koomdee, Napatrupron
Jantararoungtong, Thawinee
Prommas, Santirhat
Jinda, Pimonpan
Rachanakul, Jiratha
Nuntharadthanaphong, Nutthan
Jongjitsook, Nutcha
Puangpetch, Apichaya
Sukasem, Chonlaphat
author_sort Sukprasong, Rattanaporn
collection PubMed
description Prior knowledge of allele frequencies of cytochrome P450 polymorphisms in a population is crucial for the revision and optimization of existing medication choices and doses. In the current study, the frequency of the CYP2C9*2, CYP2C9*3, CYP2C19*2, CYP2C19*3, CYP2C19*6, CYP2C19*17, and CYP3A4 (rs4646437) alleles in a Thai population across different regions of Thailand was examined. Tests for polymorphisms of CYP2C9 and CYP3A4 were performed using TaqMan SNP genotyping assay and CYP2C19 was performed using two different methods; TaqMan SNP genotyping assay and Luminex x Tag V3. The blood samples were collected from 1205 unrelated healthy individuals across different regions within Thailand. Polymorphisms of CYP2C9 and CYP2C19 were transformed into phenotypes, which included normal metabolizer (NM), intermediate metabolizer (IM), poor metabolizer (PM), and rapid metabolizers (RM). The CYP2C9 allele frequencies among the Thai population were 0.08% and 5.27% for the CYP2C9*2 and CYP2C9*3 alleles, respectively. The CYP2C19 allele frequencies among the Thai population were 25.60%, 2.50%, 0.10%, and 1.80% for the CYP2C19*2, CYP2C19*3, CYP2C19*6, and CYP2C19*17 alleles, respectively. The allele frequency of the CYP3A4 (rs4646437) variant allele was 28.50% in the Thai population. The frequency of the CYP2C9*3 allele was significantly lower among the Northern Thai population (P < 0.001). The frequency of the CYP2C19*17 allele was significantly higher in the Southern Thai population (P < 0.001). Our results may provide an understanding of the ethnic differences in drug responses and support for the utilization of pharmacogenomics testing in clinical practice.
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spelling pubmed-81959862021-06-14 Allele frequencies of single nucleotide polymorphisms of clinically important drug-metabolizing enzymes CYP2C9, CYP2C19, and CYP3A4 in a Thai population Sukprasong, Rattanaporn Chuwongwattana, Sumonrat Koomdee, Napatrupron Jantararoungtong, Thawinee Prommas, Santirhat Jinda, Pimonpan Rachanakul, Jiratha Nuntharadthanaphong, Nutthan Jongjitsook, Nutcha Puangpetch, Apichaya Sukasem, Chonlaphat Sci Rep Article Prior knowledge of allele frequencies of cytochrome P450 polymorphisms in a population is crucial for the revision and optimization of existing medication choices and doses. In the current study, the frequency of the CYP2C9*2, CYP2C9*3, CYP2C19*2, CYP2C19*3, CYP2C19*6, CYP2C19*17, and CYP3A4 (rs4646437) alleles in a Thai population across different regions of Thailand was examined. Tests for polymorphisms of CYP2C9 and CYP3A4 were performed using TaqMan SNP genotyping assay and CYP2C19 was performed using two different methods; TaqMan SNP genotyping assay and Luminex x Tag V3. The blood samples were collected from 1205 unrelated healthy individuals across different regions within Thailand. Polymorphisms of CYP2C9 and CYP2C19 were transformed into phenotypes, which included normal metabolizer (NM), intermediate metabolizer (IM), poor metabolizer (PM), and rapid metabolizers (RM). The CYP2C9 allele frequencies among the Thai population were 0.08% and 5.27% for the CYP2C9*2 and CYP2C9*3 alleles, respectively. The CYP2C19 allele frequencies among the Thai population were 25.60%, 2.50%, 0.10%, and 1.80% for the CYP2C19*2, CYP2C19*3, CYP2C19*6, and CYP2C19*17 alleles, respectively. The allele frequency of the CYP3A4 (rs4646437) variant allele was 28.50% in the Thai population. The frequency of the CYP2C9*3 allele was significantly lower among the Northern Thai population (P < 0.001). The frequency of the CYP2C19*17 allele was significantly higher in the Southern Thai population (P < 0.001). Our results may provide an understanding of the ethnic differences in drug responses and support for the utilization of pharmacogenomics testing in clinical practice. Nature Publishing Group UK 2021-06-11 /pmc/articles/PMC8195986/ /pubmed/34117307 http://dx.doi.org/10.1038/s41598-021-90969-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Sukprasong, Rattanaporn
Chuwongwattana, Sumonrat
Koomdee, Napatrupron
Jantararoungtong, Thawinee
Prommas, Santirhat
Jinda, Pimonpan
Rachanakul, Jiratha
Nuntharadthanaphong, Nutthan
Jongjitsook, Nutcha
Puangpetch, Apichaya
Sukasem, Chonlaphat
Allele frequencies of single nucleotide polymorphisms of clinically important drug-metabolizing enzymes CYP2C9, CYP2C19, and CYP3A4 in a Thai population
title Allele frequencies of single nucleotide polymorphisms of clinically important drug-metabolizing enzymes CYP2C9, CYP2C19, and CYP3A4 in a Thai population
title_full Allele frequencies of single nucleotide polymorphisms of clinically important drug-metabolizing enzymes CYP2C9, CYP2C19, and CYP3A4 in a Thai population
title_fullStr Allele frequencies of single nucleotide polymorphisms of clinically important drug-metabolizing enzymes CYP2C9, CYP2C19, and CYP3A4 in a Thai population
title_full_unstemmed Allele frequencies of single nucleotide polymorphisms of clinically important drug-metabolizing enzymes CYP2C9, CYP2C19, and CYP3A4 in a Thai population
title_short Allele frequencies of single nucleotide polymorphisms of clinically important drug-metabolizing enzymes CYP2C9, CYP2C19, and CYP3A4 in a Thai population
title_sort allele frequencies of single nucleotide polymorphisms of clinically important drug-metabolizing enzymes cyp2c9, cyp2c19, and cyp3a4 in a thai population
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195986/
https://www.ncbi.nlm.nih.gov/pubmed/34117307
http://dx.doi.org/10.1038/s41598-021-90969-y
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