A single point mutation converts a glutaryl-7-aminocephalosporanic acid acylase into an N-acyl-homoserine lactone acylase

OBJECTIVE: To change the specificity of a glutaryl-7-aminocephalosporanic acid acylase (GCA) towards N-acyl homoserine lactones (AHLs; quorum sensing signalling molecules) by site-directed mutagenesis. RESULTS: Seven residues were identified by analysis of existing crystal structures as potential de...

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Detalles Bibliográficos
Autores principales: Murugayah, Shereen A., Evans, Gary B., Tyndall, Joel D. A., Gerth, Monica L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2021
Materias:
Original Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197700/
https://www.ncbi.nlm.nih.gov/pubmed/33891232
http://dx.doi.org/10.1007/s10529-021-03135-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197700/
https://www.ncbi.nlm.nih.gov/pubmed/33891232
http://dx.doi.org/10.1007/s10529-021-03135-9

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